Background <p>Carbapenem resistance is a global public health issue, with quorum sensing playing a key role. <i>Chromobacterium violaceum</i>, a gram-negative opportunistic pathogen, has a quorum-sensing system that contributes to its antibiotic resistance and virulence. This study analyzes the expression of quorum-sensing genes <i>cviI</i> and <i>cviR</i>, and violacein synthesis genes, in imipenem non-susceptible isolates of <i>C. violaceum</i> under different growth conditions.</p> Methods and results <p>The bacterial isolates were screened for carbapenem non-suseptibility with 1 ug/ml imipenem screen agar. Two isolates of <i>C. violaceum</i> (CD_CV48 and CD_CV64) with reduced susceptibility to imipenem were selected for the study. Both isolates exhibited minimum inhibitory concentration (MIC) values above the breakpoint for imipenem. The non-susceptible isolates were assessed for the presence of various classes of carbapenemase genes by PCR which revealed the presence of the <i>bla</i>_NDM−1 gene in one of the isolates (CD_CV48). The transcriptional response of the genes <i>cviI</i>, <i>cviR</i>, <i>vioA</i>, <i>vioB</i>, <i>vioC</i>, and <i>vioE</i> was evaluated in the isolates under different growth conditions using the Step One Plus real-time detection system. Differential expressions of <i>cviI</i>, <i>cviR</i>, <i>vioA</i>, <i>vioB</i>, <i>vioC</i>, and <i>vioE</i> genes were observed in isolates CD_CV48 and CD_CV64.</p> Conclusions <p>The findings of this study highlight the complex relationship between quorum sensing and antibiotic resistance in <i>C. violaceum.</i> The differences observed in the transcriptional profiles of genes related to quorum sensing and violacein synthesis under varying growth conditions highlight the intricacies of bacterial regulatory networks.</p>