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Background <p>Lung cancer remains the leading cause of cancer-related mortality worldwide, largely due to the development of resistance to standard chemotherapeutic agents such as cisplatin. Recent evidence has highlighted the regulatory role of long non-coding RNAs (lncRNAs) in mediating drug resistance mechanisms. In this study, we aimed to reveal the role of MCM3AP-AS1 in cisplatin-induced cell death in lung cancer cells.</p> Methods and results <p>As a result, we found that&#xa0;MCM3AP-AS1, a lncRNA previously associated with poor prognosis in various cancers, was significantly upregulated in A549 lung cancer cells following cisplatin treatment. Using RNA interference-mediated silencing of&#xa0;MCM3AP-AS1, we observed a marked increase in cell viability and a decrease in apoptosis, as determined by MTT assay, annexin V/PI flow cytometry, and acridine orange/ethidium bromide staining.</p> Conclusions <p>These findings suggest that&#xa0;MCM3AP-AS1&#xa0;may be a crucial modulator of cisplatin-induced apoptosis in lung cancer cells.</p>

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Silencing lncRNA MCM3AP-AS1 protects against cisplatin-induced cell death in A549 lung cancer cells

  • 期刊论文

  • 发表日期：

    2025年7月14日

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  • Ibrahim Bozgeyik,
  • Demet Tasdemir,
  • Esra Bozgeyik,
  • Haydar Bagis,
  • Serdar Oztuzcu,
  • Ahmet Ferudun Isik

摘要

Background

Lung cancer remains the leading cause of cancer-related mortality worldwide, largely due to the development of resistance to standard chemotherapeutic agents such as cisplatin. Recent evidence has highlighted the regulatory role of long non-coding RNAs (lncRNAs) in mediating drug resistance mechanisms. In this study, we aimed to reveal the role of MCM3AP-AS1 in cisplatin-induced cell death in lung cancer cells.

Methods and results

As a result, we found that MCM3AP-AS1, a lncRNA previously associated with poor prognosis in various cancers, was significantly upregulated in A549 lung cancer cells following cisplatin treatment. Using RNA interference-mediated silencing of MCM3AP-AS1, we observed a marked increase in cell viability and a decrease in apoptosis, as determined by MTT assay, annexin V/PI flow cytometry, and acridine orange/ethidium bromide staining.

Conclusions

These findings suggest that MCM3AP-AS1 may be a crucial modulator of cisplatin-induced apoptosis in lung cancer cells.