Scholarlink

Background <p>Cancer is one of the leading causes of death worldwide, highlighting the need to develop novel therapeutic strategies that are more effective and have fewer side effects than conventional treatments. Enzymatic cancer therapy is a promising approach due to its high specificity and minimal toxicity. Among the various enzymes, laccase, a widely used biocatalyst, has shown significant potential for anti-cancer applications due to its proliferation inhibitory properties.</p> Methods and results <p>In this study, the enzyme laccase from <i>Trametes versicolor</i> was purified by three-phase partitioning and then its cytotoxic, genotoxic and apoptotic effects on thyroid cancer (TT) and endometrial cancer (Ishikawa) cell lines were investigated. Laccase exhibited IC<sub>50</sub> values of 88.63 µM in TT cells and 1.68 µM in Ishikawa cells. The enzyme triggered apoptosis in Ishikawa cells and induced cell cycle arrest in S phase, while significantly increasing DNA damage in both cancer cell lines. Treatment with laccase led to downregulation of the anti-apoptotic gene <i>Bcl-2</i> and upregulation of the pro-apoptotic gene <i>Bax</i> and the DNA damage repair genes <i>Rad51</i> and <i>ATM</i>.</p> Conclusions <p>Our results emphasize the specific cytotoxicity and molecular mechanisms underlying the anti-cancer effect of laccase and demonstrate that laccase is capable of selectively targeting cancer cells and causing apoptosis and DNA damage. This study demonstrates the potential of laccase as a novel enzymatic therapeutic for the treatment of thyroid and endometrial cancer and warrants further investigation into its clinical application and efficacy.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Anticancer potential of purified laccase enzyme from Trametes versicolor: specific cytotoxicity against thyroid and endometrial cancer cells

  • 期刊论文

  • 发表日期：

    2025年3月14日

查看全文
  • Elif Kale Bakir,
  • Asuman Deveci Ozkan,
  • Gulay Erman,
  • Semih Isik,
  • Yonca Yuzugullu Karakus

摘要

Background

Cancer is one of the leading causes of death worldwide, highlighting the need to develop novel therapeutic strategies that are more effective and have fewer side effects than conventional treatments. Enzymatic cancer therapy is a promising approach due to its high specificity and minimal toxicity. Among the various enzymes, laccase, a widely used biocatalyst, has shown significant potential for anti-cancer applications due to its proliferation inhibitory properties.

Methods and results

In this study, the enzyme laccase from Trametes versicolor was purified by three-phase partitioning and then its cytotoxic, genotoxic and apoptotic effects on thyroid cancer (TT) and endometrial cancer (Ishikawa) cell lines were investigated. Laccase exhibited IC50 values of 88.63 µM in TT cells and 1.68 µM in Ishikawa cells. The enzyme triggered apoptosis in Ishikawa cells and induced cell cycle arrest in S phase, while significantly increasing DNA damage in both cancer cell lines. Treatment with laccase led to downregulation of the anti-apoptotic gene Bcl-2 and upregulation of the pro-apoptotic gene Bax and the DNA damage repair genes Rad51 and ATM.

Conclusions

Our results emphasize the specific cytotoxicity and molecular mechanisms underlying the anti-cancer effect of laccase and demonstrate that laccase is capable of selectively targeting cancer cells and causing apoptosis and DNA damage. This study demonstrates the potential of laccase as a novel enzymatic therapeutic for the treatment of thyroid and endometrial cancer and warrants further investigation into its clinical application and efficacy.