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<p>Phosphorus is an essential macronutrient for plant growth and development, especially in P-sensitive crops such as rapeseed (<i>Brassica napus</i>). However, the role of phosphorus (P) availability in plant disease resistance mediated by the root-associated microbiome remains poorly understood. Here, we investigated how P homeostasis regulates rapeseed resistance to <i>Sclerotinia sclerotiorum</i> through modulation of the root-associated microbiome. P deficiency significantly inhibited plant growth and increased susceptibility to <i>S. sclerotiorum</i> in multiple rapeseed ecotypes, including spring, semi‑winter, and winter types. Microbiome profiling revealed that <i>Massilia</i> was a key P-responsive biomarker genus significantly enriched under P-sufficient conditions. Both foliar application and root inoculation with <i>Massilia</i> effectively suppressed <i>S. sclerotiorum</i> infection in rapeseed. Mechanistically, <i>Massilia</i> colonization strongly activated the expression of pathogenesis‑related (<i>PR</i>) genes, antioxidant genes, and jasmonic acid (JA) signaling genes. Overall, this study establishes a P-mediated tripartite interaction linking root microbiota assembly and plant immunity. These results highlight that optimizing P supply to enrich beneficial microbes such as <i>Massilia</i> can enhance rapeseed resistance to <i>S. sclerotiorum</i>, providing a sustainable strategy for disease management.</p>

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Phosphorus availability enriches Massilia in the root microbiome to enhance resistance against Sclerotinia sclerotiorum in rapeseed

  • 期刊论文

  • 发表日期：

    2026年5月23日

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  • Xiaoxiao Dong,
  • Liang Guo,
  • Hanchen Chen

摘要

Phosphorus is an essential macronutrient for plant growth and development, especially in P-sensitive crops such as rapeseed (Brassica napus). However, the role of phosphorus (P) availability in plant disease resistance mediated by the root-associated microbiome remains poorly understood. Here, we investigated how P homeostasis regulates rapeseed resistance to Sclerotinia sclerotiorum through modulation of the root-associated microbiome. P deficiency significantly inhibited plant growth and increased susceptibility to S. sclerotiorum in multiple rapeseed ecotypes, including spring, semi‑winter, and winter types. Microbiome profiling revealed that Massilia was a key P-responsive biomarker genus significantly enriched under P-sufficient conditions. Both foliar application and root inoculation with Massilia effectively suppressed S. sclerotiorum infection in rapeseed. Mechanistically, Massilia colonization strongly activated the expression of pathogenesis‑related (PR) genes, antioxidant genes, and jasmonic acid (JA) signaling genes. Overall, this study establishes a P-mediated tripartite interaction linking root microbiota assembly and plant immunity. These results highlight that optimizing P supply to enrich beneficial microbes such as Massilia can enhance rapeseed resistance to S. sclerotiorum, providing a sustainable strategy for disease management.