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<p>Cytoplasmic male sterility (CMS), a maternally inherited trait, is a valuable approach employed for heterosis utilization in plant breeding programs. Radish is an important vegetable crop that exhibits significant heterosis. The utilization of CMS lines as female parents is essential for efficient F<sub>1</sub> hybrid seed production in radish. Among various CMS types, Ogura CMS is widely used, and its molecular mechanism has been extensively studied in radish and other <i>Brassica</i> crops, including cabbage and broccoli. However, the molecular modulator and underlying regulatory mechanism of DCGMS in radish remain elusive. In this study, six radish genotypes were identified as DCGMS with molecular markers based on the male sterility gene <i>orf463</i>. Microspore abortion of DCGMS line appeared shortly after the tetrad stage, when the tapetal cells grew abnormally large. Based on transcriptome data and bioinformatic analyses, <i>RsMYB3R-1</i> was identified as being highly expressed in stamens, and its encoded protein was predicted to target mitochondria. Yeast one-hybrid and dual-luciferase assays demonstrated that <i>RsMYB3R-1</i> bound to the <i>orf463</i> promoter. Transient overexpression of <i>RsMYB3R-1</i> in radish cotyledons could increase the expression level of <i>orf463</i>. This result may cause mitochondrial energy production dysfunction and abnormal pollen development in the DCGMS lines. Taken together, our findings elucidate that the RsMYB3R-1-<i>orf463</i> module plays a pivotal role in mediating pollen abortion in the DCGMS line of radish. These results not only offer novel insights into the nucleus–mitochondria interaction, but also establish a foundation for deciphering the molecular mechanisms underlying DCGMS male sterility, thereby facilitating the efficient utilization of heterosis in radish breeding.</p>

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RsMYB3R-1 confers DCGMS male sterility by regulating the expression of orf463 gene in radish (Raphanus sativus L.)

  • 期刊论文

  • 发表日期：

    2026年5月25日

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  • Bingshuang Li,
  • Nannan Liao,
  • Xiaoli Zhang,
  • Yan Wang,
  • Liang Xu,
  • Junhui Dong,
  • Feng Cui,
  • Sen Chen,
  • Yinbo Ma,
  • Liwang Liu

摘要

Cytoplasmic male sterility (CMS), a maternally inherited trait, is a valuable approach employed for heterosis utilization in plant breeding programs. Radish is an important vegetable crop that exhibits significant heterosis. The utilization of CMS lines as female parents is essential for efficient F1 hybrid seed production in radish. Among various CMS types, Ogura CMS is widely used, and its molecular mechanism has been extensively studied in radish and other Brassica crops, including cabbage and broccoli. However, the molecular modulator and underlying regulatory mechanism of DCGMS in radish remain elusive. In this study, six radish genotypes were identified as DCGMS with molecular markers based on the male sterility gene orf463. Microspore abortion of DCGMS line appeared shortly after the tetrad stage, when the tapetal cells grew abnormally large. Based on transcriptome data and bioinformatic analyses, RsMYB3R-1 was identified as being highly expressed in stamens, and its encoded protein was predicted to target mitochondria. Yeast one-hybrid and dual-luciferase assays demonstrated that RsMYB3R-1 bound to the orf463 promoter. Transient overexpression of RsMYB3R-1 in radish cotyledons could increase the expression level of orf463. This result may cause mitochondrial energy production dysfunction and abnormal pollen development in the DCGMS lines. Taken together, our findings elucidate that the RsMYB3R-1-orf463 module plays a pivotal role in mediating pollen abortion in the DCGMS line of radish. These results not only offer novel insights into the nucleus–mitochondria interaction, but also establish a foundation for deciphering the molecular mechanisms underlying DCGMS male sterility, thereby facilitating the efficient utilization of heterosis in radish breeding.