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<p>Pumpkin (<i>Cucurbita pepo</i> L.) is an economically important vegetable crop, and fruit size is a key agronomic trait determining its commercial value. Quantitative trait loci (QTLs) controlling fruit length (FL) have been widely studied in other cucurbit species, but few such studies have been reported in pumpkin. In this study, a short-fruited line Jin-GL was crossed with a long-fruited line Nei. The F₂ population displayed continuous fruit-length variation (5–25&#xa0;cm). When classified using the F₁ mean FL (approximately 11&#xa0;cm) as a threshold, the F₂ individuals showed a segregation of 191 short vs. 79 long (191:79; close to 3:1), supporting the presence of a major-effect locus. Then, we fine mapped the locus, designated <i>Cpfl17.1</i>, into a 21.6&#xa0;kb interval. To construct the near-isogenic lines (NILs), three generations of backcrossing the F₁ to each parent were developed. The flanking markers InDel17-8 and InDel17-13 were used for foreground selection, and a total of 101 InDel markers was selected with good polymorphism between two parental lines for background selection which had relatively uniform distribution across 20 chromosomes. The proportion of recurrent parent genome (PRPG) of the NILs is larger than 98%. And the FL phenotypes showed significantly differ between the NILs and recurrent parent. Comparison with previously reported fruit-length QTLs showed no positional overlap with <i>Cpfl17.1</i>, suggesting that it may represent an independent locus. Our results demonstrate that <i>Cpfl17.1</i> is a new breeding target for marker-assisted selection, which will facilitate precise introgression of fruit shape–related alleles and accelerate the development of new varieties with improved fruit shape.</p>

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Fine mapping of a novel fruit-length locus Cpfl17.1 and development of near-isogenic lines by marker-assisted selection in Cucurbita pepo L.

  • 期刊论文

  • 发表日期：

    2026年3月6日

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  • Liuqing Sun,
  • Yapei Sun,
  • Xiyan Qiu,
  • Wanlu Chen,
  • Kiros Gebretsadik,
  • Ying Duan,
  • Qunfeng Lou,
  • Changlin Wang,
  • Kailiang Bo

摘要

Pumpkin (Cucurbita pepo L.) is an economically important vegetable crop, and fruit size is a key agronomic trait determining its commercial value. Quantitative trait loci (QTLs) controlling fruit length (FL) have been widely studied in other cucurbit species, but few such studies have been reported in pumpkin. In this study, a short-fruited line Jin-GL was crossed with a long-fruited line Nei. The F₂ population displayed continuous fruit-length variation (5–25 cm). When classified using the F₁ mean FL (approximately 11 cm) as a threshold, the F₂ individuals showed a segregation of 191 short vs. 79 long (191:79; close to 3:1), supporting the presence of a major-effect locus. Then, we fine mapped the locus, designated Cpfl17.1, into a 21.6 kb interval. To construct the near-isogenic lines (NILs), three generations of backcrossing the F₁ to each parent were developed. The flanking markers InDel17-8 and InDel17-13 were used for foreground selection, and a total of 101 InDel markers was selected with good polymorphism between two parental lines for background selection which had relatively uniform distribution across 20 chromosomes. The proportion of recurrent parent genome (PRPG) of the NILs is larger than 98%. And the FL phenotypes showed significantly differ between the NILs and recurrent parent. Comparison with previously reported fruit-length QTLs showed no positional overlap with Cpfl17.1, suggesting that it may represent an independent locus. Our results demonstrate that Cpfl17.1 is a new breeding target for marker-assisted selection, which will facilitate precise introgression of fruit shape–related alleles and accelerate the development of new varieties with improved fruit shape.