Scholarlink

<p>The presence of cadmium (Cd) in rice poses a significant health risk to consumers, highlighting the urgency of breeding rice varieties with low Cd accumulation. To identify genetic resources and potential genes for developing such rice varieties, a comprehensive genome-wide association study (GWAS) was conducted on 158 rice varieties, which tested between 2021 and 2023 in low cadmium accumulation testing framework, to identify candidate genes associated with cadmium content in brown rice. Based on their parental origin and genetic population structure analysis, we categorized these 158 varieties into four subgroups: Luohong, <i>lcd1</i>, intermediate and early indica series. Specifically, the four subgroups of low cadmium varieties were breeded based on <i>OsNramp5</i> mutants Luohong 3A/4A, <i>lcd1</i>, Lian 1S and Shaoxiang 100, respectively. GWAS analysis identified sixteen loci significantly associated with cadmium content, twelve of which showed consistent associations across multiple environments, these loci were mapped to chromosomes 1, 2, 5, 7, 11, and 12, suggesting their potential for further fine mapping and functional validation. Through gene function annotation analysis, candidate genes related to cadmium content in these loci were identified, including <i>Os05 g0382200</i>, <i>Os07 g0232800</i> (<i>OsZIP8</i>), <i>Os07 g0232900</i> (<i>OsHMA3</i>), <i>Os07 g0257200</i> (<i>OsNramp5</i>), <i>Os07 g0258400</i> (<i>OsNramp1</i>), <i>Os12 g0512100</i>, <i>Os12 g0512700</i>, and <i>Os12 g0514000</i>. These genes are implicated in the absorption, transport, and accumulation of heavy metals, particularly cadmium. Haplotype analysis of key genes <i>OsZIP8</i>, <i>OsHMA3</i>, <i>OsNramp5</i>, and <i>OsNramp1</i> identified specific low-cadmium dominant haplotypes. Notably, <i>OsHMA3</i>-Hap2 (GC), <i>OsNramp5</i>-Hap1 (DEL), and <i>OsNramp1</i>-Hap1 (DEL) were associated with Luohong-origin varieties, while <i>OsHMA3</i>-Hap1 (AC), <i>OsNramp5</i>-Hap2 (AA), and <i>OsNramp1</i>-Hap2 (GGG) were linked to <i>lcd1</i>-origin varieties. Overall, this study illustrated the genetic basis for breeding low-cadmium rice varieties and provided candidate loci to develop molecular markers to enhance food safety through reduced heavy metal content.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Navigating the genetic basis of cadmium accumulation: a comprehensive analysis of low-cadmium accumulation rice

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月2日

查看全文
  • Deyi Shao,
  • Lixin Yin,
  • Jian Zhao,
  • Zhengliang Luo,
  • Weiguo Li,
  • Yongka Wang,
  • Xiangjie Liu,
  • Bingchuan Tian,
  • Xiaobo Long,
  • Hexing Yin,
  • Kun Zhou

摘要

The presence of cadmium (Cd) in rice poses a significant health risk to consumers, highlighting the urgency of breeding rice varieties with low Cd accumulation. To identify genetic resources and potential genes for developing such rice varieties, a comprehensive genome-wide association study (GWAS) was conducted on 158 rice varieties, which tested between 2021 and 2023 in low cadmium accumulation testing framework, to identify candidate genes associated with cadmium content in brown rice. Based on their parental origin and genetic population structure analysis, we categorized these 158 varieties into four subgroups: Luohong, lcd1, intermediate and early indica series. Specifically, the four subgroups of low cadmium varieties were breeded based on OsNramp5 mutants Luohong 3A/4A, lcd1, Lian 1S and Shaoxiang 100, respectively. GWAS analysis identified sixteen loci significantly associated with cadmium content, twelve of which showed consistent associations across multiple environments, these loci were mapped to chromosomes 1, 2, 5, 7, 11, and 12, suggesting their potential for further fine mapping and functional validation. Through gene function annotation analysis, candidate genes related to cadmium content in these loci were identified, including Os05 g0382200, Os07 g0232800 (OsZIP8), Os07 g0232900 (OsHMA3), Os07 g0257200 (OsNramp5), Os07 g0258400 (OsNramp1), Os12 g0512100, Os12 g0512700, and Os12 g0514000. These genes are implicated in the absorption, transport, and accumulation of heavy metals, particularly cadmium. Haplotype analysis of key genes OsZIP8, OsHMA3, OsNramp5, and OsNramp1 identified specific low-cadmium dominant haplotypes. Notably, OsHMA3-Hap2 (GC), OsNramp5-Hap1 (DEL), and OsNramp1-Hap1 (DEL) were associated with Luohong-origin varieties, while OsHMA3-Hap1 (AC), OsNramp5-Hap2 (AA), and OsNramp1-Hap2 (GGG) were linked to lcd1-origin varieties. Overall, this study illustrated the genetic basis for breeding low-cadmium rice varieties and provided candidate loci to develop molecular markers to enhance food safety through reduced heavy metal content.