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<p>Taigu genic male-sterile wheat, containing the dominant gene <i>male sterile</i> (<i>Ms</i>) <i>2</i>, shows completely male-sterility in different genetic background and under various environments. The development of Aibai wheat with tightly linked <i>Ms2</i> and <i>Reduced height</i> (<i>Rht</i>)<i>-D1c</i>, offers possibility for identifying the male-sterile plants by investigation of reduced plant height. However, due to the extreme dwarfism of Aibai wheat caused by <i>Rht-D1c</i>, it inhabits a shadowed microenvironment caused by its fertile sibling plants. This results in delayed maturation of sterile plant progeny. In order to develop a novel germplasm with new visible marker for sorting male sterile plants conferred by <i>Ms2</i>, a binary vector containing <i>Bar</i>, <i>Ms2</i>, <i>Rht-D1b</i>, and <i>DsRed</i> driven by the aleurone-specific promoter <i>Ltp2</i> was constructed and introduced into the wheat Fielder using <i>Agrobacterium</i>-mediated transformation in this study. After investigation of the fertility, plant height and seed fluorescence of positive transgenic wheat plants, a line exhibited semi-dwarf male sterility, which could be reliably identified by the aleurone-specifically expressed red fluorescence in seeds, serving as a genetically stable reporter. Therefore, this study provides a novel male-sterile wheat that offers a powerful tool for hybrid seed production and facilitates genetic improvement in wheat through recurrent selection.</p>

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Development of a semi-dwarf dominant genic male sterile wheat with seed red fluorescence as a visible marker

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Ke Zheng,
  • Xiaonan Zhou,
  • Ningyao Xu,
  • Demei Liu,
  • Haiqing Wang

摘要

Taigu genic male-sterile wheat, containing the dominant gene male sterile (Ms) 2, shows completely male-sterility in different genetic background and under various environments. The development of Aibai wheat with tightly linked Ms2 and Reduced height (Rht)-D1c, offers possibility for identifying the male-sterile plants by investigation of reduced plant height. However, due to the extreme dwarfism of Aibai wheat caused by Rht-D1c, it inhabits a shadowed microenvironment caused by its fertile sibling plants. This results in delayed maturation of sterile plant progeny. In order to develop a novel germplasm with new visible marker for sorting male sterile plants conferred by Ms2, a binary vector containing Bar, Ms2, Rht-D1b, and DsRed driven by the aleurone-specific promoter Ltp2 was constructed and introduced into the wheat Fielder using Agrobacterium-mediated transformation in this study. After investigation of the fertility, plant height and seed fluorescence of positive transgenic wheat plants, a line exhibited semi-dwarf male sterility, which could be reliably identified by the aleurone-specifically expressed red fluorescence in seeds, serving as a genetically stable reporter. Therefore, this study provides a novel male-sterile wheat that offers a powerful tool for hybrid seed production and facilitates genetic improvement in wheat through recurrent selection.