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<p>Tiller number is a key determinant of the number of spikes per plant, significantly influencing yield. Here, we identify and characterize a novel tiller inhibition line, N2496. Using an F<sub>2</sub> segregating population derived from crossing N2496 and CN16, we mapped this locus. The F<sub>1</sub> line demonstrated a high number of tillers, while the F<sub>2</sub> population exhibited segregated ratios of 3:1 in tiller number. BSR-Seq analysis indicated that only one locus controls tiller number, located on chromosome 2B (Chr. 2B). This genetic analysis confirmed the presence of a single recessive locus controlling the tiller inhibition trait within this population. Subsequently, we constructed a genetic map on Chr. 2B using a wheat 55&#xa0;K single nucleotide polymorphism array. By combining recombinant analysis with the genotype and phenotype of the F<sub>2-3</sub> family, we identified and named a major and novel locus, <i>tiller inhibition gene</i> (<i>tin7</i>), mapped within a 2.43&#xa0;cM interval. The influence of <i>tin7</i> was verified across six different background populations all sharing N2496 as a common parent. Using new recombinant lines from these six populations, we further narrowed down the interval of <i>tin7</i> to a genetic interval of 2.08&#xa0;cM. Analysis of thousand grain weight and grain-related traits suggests that by regulating tiller number, <i>tin7</i> holds the potential to increase yield in wheat. Our research provides access to a novel tiller number locus and available markers for regulating tiller number, which could be used in developing new cultivars with an optimal number of tillers.</p>

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Identification and validation of a novel tiller inhibition locus (tin7) on chromosome 2BL in wheat

  • 期刊论文

  • 发表日期：

    2025年5月1日

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  • Shuai Hou,
  • Yuzhou Mou,
  • Haojie Li,
  • Caixia Li,
  • Zhiqiang Wang,
  • Yu Lin,
  • Yueyue Liu,
  • Yaxi Liu

摘要

Tiller number is a key determinant of the number of spikes per plant, significantly influencing yield. Here, we identify and characterize a novel tiller inhibition line, N2496. Using an F2 segregating population derived from crossing N2496 and CN16, we mapped this locus. The F1 line demonstrated a high number of tillers, while the F2 population exhibited segregated ratios of 3:1 in tiller number. BSR-Seq analysis indicated that only one locus controls tiller number, located on chromosome 2B (Chr. 2B). This genetic analysis confirmed the presence of a single recessive locus controlling the tiller inhibition trait within this population. Subsequently, we constructed a genetic map on Chr. 2B using a wheat 55 K single nucleotide polymorphism array. By combining recombinant analysis with the genotype and phenotype of the F2-3 family, we identified and named a major and novel locus, tiller inhibition gene (tin7), mapped within a 2.43 cM interval. The influence of tin7 was verified across six different background populations all sharing N2496 as a common parent. Using new recombinant lines from these six populations, we further narrowed down the interval of tin7 to a genetic interval of 2.08 cM. Analysis of thousand grain weight and grain-related traits suggests that by regulating tiller number, tin7 holds the potential to increase yield in wheat. Our research provides access to a novel tiller number locus and available markers for regulating tiller number, which could be used in developing new cultivars with an optimal number of tillers.