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<p>Exploring the molecular mechanism underlying plant architecture and breeding new varieties suitable for mechanized harvesting are primary objectives for rapeseed breeders in China. However, few genes controlling plant architecture have been cloned in <i>Brassica napus</i>. In this study, SX3, a scattered-bud <i>B. napus</i> line with a dwarf and compact plant architecture, was characterized. To identify the genes underlying bud arrangement, plant height and branch angle, segregating populations were constructed by crossing SX3 with two clustered-bud lines with a tall and loose plant architecture. Genetic analysis revealed that the scattered-bud trait (SBT) was controlled by a single dominant gene, <i>BnaSBT</i>. <i>BnaSBT</i> is likely a pleiotropic gene that simultaneously controls plant height and branch angle. Using BSA-seq analysis, <i>BnaSBT</i> was mapped to a 4.15&#xa0;Mb region on ChrA10. Owing to the lack of recombinants within this region, it was infeasible to finely map <i>BnaSBT</i>. RNA-seq analysis of BC<sub>2</sub> plants with contrasting inflorescence and plant architectures revealed that the upregulation of genes involved in amino acid and lipid metabolism and genes encoding MADS-box transcription factors is related to the the phenotype of SX3. These findings together with comparative sequencing indicated that <i>BnaA10.SEP1</i>, <i>BnaA10.AGL15</i>, <i>BnaA10.GLN1-4</i> and <i>BnaA10.AGP15</i> are candidate genes for <i>BnaSBT</i>. Markers closely linked to the scattered-bud trait were developed for selecting dwarf and compact plants. These findings provide molecular markers and germplasms for breeding new varieties with ideal plant types and lay a theoretical foundation for cloning key genes and elucidating the genetic basis of inflorescence and plant architectures in <i>B. napus.</i></p>

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Mapping and molecular marker development for the BnaSBT gene controlling inflorescence and plant architectures in B. napus

  • 期刊论文

  • 发表日期：

    2025年4月15日

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  • Meng Jiang,
  • Jingming Li,
  • Yingying Huang,
  • Baolong Tao,
  • Lumei Wu,
  • Junlin Chen,
  • Lun Zhao,
  • Bin Yi,
  • Chaozhi Ma,
  • Jinxing Tu,
  • Jinxiong Shen,
  • Tingdong Fu,
  • Jing Wen

摘要

Exploring the molecular mechanism underlying plant architecture and breeding new varieties suitable for mechanized harvesting are primary objectives for rapeseed breeders in China. However, few genes controlling plant architecture have been cloned in Brassica napus. In this study, SX3, a scattered-bud B. napus line with a dwarf and compact plant architecture, was characterized. To identify the genes underlying bud arrangement, plant height and branch angle, segregating populations were constructed by crossing SX3 with two clustered-bud lines with a tall and loose plant architecture. Genetic analysis revealed that the scattered-bud trait (SBT) was controlled by a single dominant gene, BnaSBT. BnaSBT is likely a pleiotropic gene that simultaneously controls plant height and branch angle. Using BSA-seq analysis, BnaSBT was mapped to a 4.15 Mb region on ChrA10. Owing to the lack of recombinants within this region, it was infeasible to finely map BnaSBT. RNA-seq analysis of BC2 plants with contrasting inflorescence and plant architectures revealed that the upregulation of genes involved in amino acid and lipid metabolism and genes encoding MADS-box transcription factors is related to the the phenotype of SX3. These findings together with comparative sequencing indicated that BnaA10.SEP1, BnaA10.AGL15, BnaA10.GLN1-4 and BnaA10.AGP15 are candidate genes for BnaSBT. Markers closely linked to the scattered-bud trait were developed for selecting dwarf and compact plants. These findings provide molecular markers and germplasms for breeding new varieties with ideal plant types and lay a theoretical foundation for cloning key genes and elucidating the genetic basis of inflorescence and plant architectures in B. napus.