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<p>Diabetic cardiomyopathy (DCM) is a diabetes mellitus-induced pathophysiological condition caused by unfavorable myocardial sequelae, with more severe cardiac dysfunction observed in patients with diabetes than in those without diabetes. Recently, ferroptosis has been implicated in DCM; however, its role in DCM remains incompletely elucidated. This study was conducted to examine the impact of mitochondrial ubiquitin ligase (MITOL/March5) and the mitophagy receptor Parkin on DCM-induced cardiac dysfunction, as well as the effect of ferritinophagy. Wild-type and <i>db/db</i> mice were fed normal chow or a high-fat diet and subjected to streptozotocin treatment. Cardiac geometry and function, as well as ferroptosis-related biomarkers, were assessed upon completion of experiments. Our findings revealed that DCM induced notable alterations in cardiac geometry by increasing myocardial fibrosis and ferroptosis, involving increased reactive oxygen species production and lipid accumulation. Nuclear receptor coactivator 4 (NCOA4)-related ferroptosis was significantly activated, whereas Parkin-dependent mitophagy was dramatically inhibited. However, adeno-associated virus (AAV)-MITOL treatment markedly attenuated cardiac dysfunction and ferroptosis with those in the DCM group. Furthermore, Ad Parkin alleviated NCOA4-mediated ferroptosis and suppressed myocardial apoptosis compared with those in the high-glucose/high-fat group. Our in vitro analysis demonstrated that MITOL inhibition compromised cardiomyocyte function and elicited mitochondrial injury and lipid peroxidation, the effects of which were negated by Parkin activation. This study underscores the pivotal protective role of the MITOL/Parkin signaling pathway against DCM-induced cardiac dysfunction in NCOA4-mediated ferritinophagy.</p>

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Inhibition of NCOA4-mediated ferritinophagy improves cardiac remodeling in diabetic cardiomyopathy via MITOL/parkin signaling

  • 期刊论文

  • 发表日期：

    2026年4月11日

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  • Linhe Lu,
  • Yang Liu,
  • Yalan Shao,
  • Xiang Xiong,
  • Mengen Zhai,
  • Jian Yang,
  • Lifang Yang

摘要

Diabetic cardiomyopathy (DCM) is a diabetes mellitus-induced pathophysiological condition caused by unfavorable myocardial sequelae, with more severe cardiac dysfunction observed in patients with diabetes than in those without diabetes. Recently, ferroptosis has been implicated in DCM; however, its role in DCM remains incompletely elucidated. This study was conducted to examine the impact of mitochondrial ubiquitin ligase (MITOL/March5) and the mitophagy receptor Parkin on DCM-induced cardiac dysfunction, as well as the effect of ferritinophagy. Wild-type and db/db mice were fed normal chow or a high-fat diet and subjected to streptozotocin treatment. Cardiac geometry and function, as well as ferroptosis-related biomarkers, were assessed upon completion of experiments. Our findings revealed that DCM induced notable alterations in cardiac geometry by increasing myocardial fibrosis and ferroptosis, involving increased reactive oxygen species production and lipid accumulation. Nuclear receptor coactivator 4 (NCOA4)-related ferroptosis was significantly activated, whereas Parkin-dependent mitophagy was dramatically inhibited. However, adeno-associated virus (AAV)-MITOL treatment markedly attenuated cardiac dysfunction and ferroptosis with those in the DCM group. Furthermore, Ad Parkin alleviated NCOA4-mediated ferroptosis and suppressed myocardial apoptosis compared with those in the high-glucose/high-fat group. Our in vitro analysis demonstrated that MITOL inhibition compromised cardiomyocyte function and elicited mitochondrial injury and lipid peroxidation, the effects of which were negated by Parkin activation. This study underscores the pivotal protective role of the MITOL/Parkin signaling pathway against DCM-induced cardiac dysfunction in NCOA4-mediated ferritinophagy.