<p>In electric vehicles, overheating issues caused by high-power consumption and ensuring temperature uniformity necessitate an effective battery thermal management system (BTMS). The inadequacy of passive methods in BTMS has increased interest in active methods. Pulsating airflow, one of the active methods, has significant potential in improving heat transfer. Research investigating the effects of pulsating airflow on BTMS is relatively new, and the topic is still in its developmental stages. Therefore, this study focuses on investigating the effect of pulsating airflow on enhanced heat transfer and temperature uniformity in BTMS. A numerical study was conducted using CFD-based ANSYS Fluent software. A total of 30 cylindrical Li-ion battery cells (5s6p) were arranged in an inline configuration within the module. The effects of Reynolds number (6000 ≤ Re ≤ 18,000), pulsating amplitude (0.3 ≤ <i>A</i> ≤ 0.8), and Strouhal number (2 ≤ St ≤ 8) on the BTMS were investigated. Instantaneous and average temperature values, Nusselt number (Nu), heat transfer improvement rate (<i>η</i>), pressure drop (Δ<i>P</i>), pumping power (<i>W</i>_power), and thermohydraulic performance (THP) were calculated for different parameters. The findings indicated that pulsating flow provided a more uniform temperature distribution within the module compared to steady flow. Increasing the Re increased heat transfer and pressure drop for both steady and pulsating flow. Higher heat transfer was achieved at lower pulsating amplitudes, and a critical Strouhal number (St = 4) was identified for heat transfer. At Re = 18,000, <i>A</i> = 0.8, and St = 4, heat transfer increased by 1.59 times compared to the steady-state condition, while pressure drop increased by 42.26%. At Re = 12,000, <i>A</i> = 0.3, and St = 4, the difference between the highest and lowest temperatures among the first-row cells was found to be Δ<i>T</i> = 4.85&#xa0;K, which is below the critical temperature value (≤ 5&#xa0;°C).</p>