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<p>This study investigates the physicochemical properties and antibacterial activity of hydroxyapatite (HA) nanoparticles synthesized using <i>Basella alba</i> mucilage (BAM) as a natural template and coated with <i>Stephania pierrei</i> (<i>S. pierrei</i>) extract. HA composites were prepared via the sol-gel method with varying BAM concentrations (0–25 wt%) to optimize material properties. <i>S. pierrei</i> tuber and leaf extracts were then incorporated, forming functionalized <i>S. pierrei</i>/BAM-HA materials. Characterization via X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), and N<sub>2</sub> adsorption/desorption confirmed the formation of biphasic calcium phosphate structures with enhanced surface area and reduced particle sizes. The 5 wt% BAM composite exhibited the highest surface area (17.59 m<sup>2</sup>/g) and smallest particle size (6.57 nm). Antibacterial activity was evaluated against <i>Pseudomonas aeruginosa</i>, <i>Bacillus cereus</i>, <i>Staphylococcus aureus</i>, and <i>Bacillus subtilis</i> using the agar well diffusion method. The <i>S. pierrei</i>/BAM-HA composites showed selective antibacterial effects, particularly against <i>Pseudomonas aeruginosa</i> and <i>Bacillus cereus</i>. Notably, 4.0<i>S. pierrei</i>-L/BAM-HA exhibited the strongest activity against <i>Bacillus cereus</i> (MIC and MBC: 4 mg/mL). This research highlights the potential of <i>S. pierrei</i>/BAM-HA composites as antibacterial coatings for implants and bone tissue engineering, while suggesting that further modifications may be needed to enhance antifungal properties.</p> Graphical Abstract <p></p>

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Antibacterial Performance of Stephania pierrei-Functionalized Hydroxyapatite Nanoparticles Synthesized Using Basella alba Mucilage as a Template

  • 期刊论文

  • 发表日期：

    2025年8月9日

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  • Sirilak Kamonwannasit,
  • Orrasa Prasitnok,
  • Khongvit Prasitnok,
  • Pongtanawat Khemthong,
  • Saran Youngjan,
  • Teera Butburee,
  • Pantita Promsrikaew,
  • Nanthicha Buahongsa,
  • Jiyapa Sripirom,
  • Agarat Kamcharoen,
  • Piaw Phatai

摘要

This study investigates the physicochemical properties and antibacterial activity of hydroxyapatite (HA) nanoparticles synthesized using Basella alba mucilage (BAM) as a natural template and coated with Stephania pierrei (S. pierrei) extract. HA composites were prepared via the sol-gel method with varying BAM concentrations (0–25 wt%) to optimize material properties. S. pierrei tuber and leaf extracts were then incorporated, forming functionalized S. pierrei/BAM-HA materials. Characterization via X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, transmission electron microscopy (TEM), and N2 adsorption/desorption confirmed the formation of biphasic calcium phosphate structures with enhanced surface area and reduced particle sizes. The 5 wt% BAM composite exhibited the highest surface area (17.59 m2/g) and smallest particle size (6.57 nm). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus aureus, and Bacillus subtilis using the agar well diffusion method. The S. pierrei/BAM-HA composites showed selective antibacterial effects, particularly against Pseudomonas aeruginosa and Bacillus cereus. Notably, 4.0S. pierrei-L/BAM-HA exhibited the strongest activity against Bacillus cereus (MIC and MBC: 4 mg/mL). This research highlights the potential of S. pierrei/BAM-HA composites as antibacterial coatings for implants and bone tissue engineering, while suggesting that further modifications may be needed to enhance antifungal properties.

Graphical Abstract