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<p>Freeze-drying plays an essential role in creating human serum albumin nanocolloid kits, necessitating the careful choice of stabilizing agents to preserve structural integrity and radiochemical purity after technetium-99&#xa0;m labeling. This study used trehalose, frequently employed to stabilize proteins during lyophilization, and evaluated its role as a cryoprotectant relative to glucose. Trehalose at 15&#xa0;mg/mL showed favorable particle size, zeta potential, and radiochemical purity, with stability comparable to glucose. It also offered protection against structural damage. These results support the use of trehalose as a potential alternative cryoprotectant in commercial kits, making it appropriate for use in radiopharmaceutical applications.</p> Graphical Abstract <p></p>

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Comparative study of trehalose and glucose in freeze-dried human serum albumin nanocolloids: implications for technetium-99m radiolabeling

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • Ratna Dini Haryuni,
  • Wening Lestari,
  • Mochammad Dedy Dwi Setyawan,
  • Ega Pratama Hadinoto,
  • Veronika Yulianti Susilo,
  • Amal Rezka Putra,
  • Ahsanal Fikri,
  • Ligwina Dita Pertiwi,
  • Alfian Mahardika Forentin,
  • Sri Setiyowati,
  • Grace Tjungirai Sulungbudi,
  • Ratu Ralna Ismuha,
  • Sumandi Juliyanto

摘要

Freeze-drying plays an essential role in creating human serum albumin nanocolloid kits, necessitating the careful choice of stabilizing agents to preserve structural integrity and radiochemical purity after technetium-99 m labeling. This study used trehalose, frequently employed to stabilize proteins during lyophilization, and evaluated its role as a cryoprotectant relative to glucose. Trehalose at 15 mg/mL showed favorable particle size, zeta potential, and radiochemical purity, with stability comparable to glucose. It also offered protection against structural damage. These results support the use of trehalose as a potential alternative cryoprotectant in commercial kits, making it appropriate for use in radiopharmaceutical applications.

Graphical Abstract