<p>It is well-established that molecular recognition concerning carrier agents is key in transporting and diffusing biologically active molecules across cell membranes. In this context, synthetic facilitated transport membranes (<b>FTMs</b>) have been utilized to extract and transport lactic acid (LA), a valuable compound with various industrial applications. We applied a PVDF-based Polymeric Inclusion Membrane (PIM) incorporating Aliquat 336 as the carrier, prepared via the standard phase inversion method. The resulting PIMs were subsequently characterized using infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and ATG-ATD thermal analysis. Macroscopic parameters, such as permeability (<Emphasis Type="BoldItalic">P</Emphasis>) and initial flux (<Emphasis Type="BoldItalic">J</Emphasis>_{<Emphasis Type="BoldItalic">0</Emphasis>}), were evaluated to characterize membrane performance, while microscopic parameters, including the association constant (<Emphasis Type="BoldItalic">K</Emphasis>_{<Emphasis Type="BoldItalic">ass</Emphasis>}) and the apparent diffusion coefficient (<Emphasis Type="BoldItalic">D*</Emphasis>), were determined to describe the transport mechanism of LA molecules through the membrane phase. These measurements were systematically performed by studying the effects of initial LA concentration (0.4, 0.5, 0.8, and 1&#xa0;M), Aliquat 336 content in the membrane (20, 30, and 40 wt%), pH of the medium (2, 4, and 6), and temperature (298, 303, and 305&#xa0;K). In addition, activation energy and thermodynamic parameters (<Emphasis Type="BoldItalic">E</Emphasis>_{<Emphasis Type="BoldItalic">a</Emphasis>}, <Emphasis Type="BoldItalic">ΔH</Emphasis>^{<Emphasis Type="BoldItalic">≠</Emphasis>}, <Emphasis Type="BoldItalic">ΔS</Emphasis>^{<Emphasis Type="BoldItalic">≠</Emphasis>}, and <Emphasis Type="BoldItalic">ΔH</Emphasis>_{<Emphasis Type="BoldItalic">th</Emphasis>}) were determined to explain the kinetic and energetic aspects governing the mechanisms of the processes. Data demonstrate that PIM-Aliquat 336 (30 wt%) displayed high effectiveness and performance in the extraction and recovery of the value-added compound LA, particularly in a medium with a pH of 6 with permeability (<Emphasis Type="BoldItalic">P</Emphasis>) of 35.926 × 10⁻⁷ cm²·s⁻¹ and initial flux (<Emphasis Type="BoldItalic">J</Emphasis>_{<Emphasis Type="BoldItalic">0</Emphasis>} ) of 16.750 × 10⁻⁵ mmol·cm⁻²·s⁻¹. These transport processes, generally directed by structural kinetic control within the PIM, remained efficient even at low temperatures, with activation energy (<b>E</b>_<b>a</b>) of 9.311 ∓ 0.199&#xa0;kJ·mol⁻¹ and enthalpy of association (<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\:{\varvec{\varDelta\:}\varvec{H}}_{\varvec{a}\varvec{s}\varvec{s}}^{\ne\:}\)</EquationSource> </InlineEquation>) of 6.833 ∓ 0.199&#xa0;kJ·mol⁻¹. Such characteristics make PIM- Aliquat 336 a promising membrane for the extraction and purification of temperature-sensitive biological compounds.</p>