<p>The rapid development of nanotechnology has led to the continuous increase of wastewater containing metal oxide particles as well as organic dyes. Coagulation/flocculation is one of the most extensively used methods to remove these contaminants. This present study focuses on preparing a thermoresponsive graft polymer Chitosan – poly (N-isopropylacrylamide) (CS-PNIPAM) as a flocculant for the removal of metal oxide nanoparticles (TiO₂, ZnO, Fe₃O₄) and organic dyes (methyl orange, methylene blue). In general, the lower critical solution temperature (LCST) of the polymer was increased along with the increase of mercaptoacetic acid (MAA) as well as chitosan (CS) amount. The CS-PNIPAM graft polymer with 10% chitosan which showed LCST of 38.3 ^oC and the highest grafting yield of 88.5% was chosen for further studies. The zeta potentials of the polymers changed from nearly 5 mV for PNIPAM to -23 mV for PNI-COOH and 51 mV for CS-PNIPAM. For the flocculation experiments, CS-PNIPAM showed high removal efficacy for all three metal oxide particles. The quantitative measurement of iron concentration presented a removal percentage of about 95% after 30&#xa0;min of exposure at 50 ^oC. The flocculation efficiency was very similar at pH 5 and 7, but significantly lower at pH 9, indicating the pH dependency of the flocculation performance due to the presence of CS in the polymer structure. The organic dye flocculation showed that CS-PNIPAM graft polymer is effective on methyl orange (MO, 84% removal) but not methylene blue (MB, 20% removal). This could be due to the highly positive charge of the polymer, thus inducing the charge neutralization process for MO removal. The flocculation mechanism was also corroborated through flocs analysis. These results exhibited the potential of utilizing thermoresponsive CS-PNIPAM graft polymer as a flocculant for metal oxide particles and anionic dyes.</p>