<p>Existing research has explored the development of adolescents’ character strengths and their influencing factors. However, it is understudied whether adolescents’ character strengths exhibit distinct latent profiles, especially whether these profiles change with age during early adolescence, as well as the role of parental acceptance and rejection on this transition. The present longitudinal study examined this issue. A total of 569 adolescents (<i>M</i>_age = 13.51, <i>SD</i> = 0.87, at T1; 257 girls) were included in the analysis, with three waves of data collected six months apart in a longitudinal design. The results revealed that the three latent profiles of low, moderate, and high character strengths were identified in adolescents at T1, T2, and T3. Adolescents’ character strengths demonstrated relatively high stability. From T1 to T3, adolescents in the low, moderate, and high character strength groups were more likely to remain in their original groups. Among adolescents who experienced transitions over time, shifts were more likely to favor positive rather than negative. Parental acceptance consistently and positively predicted transitions in character strength across all groups. Differently, the predictive effect of parental rejection on the transitions of adolescents’ character strengths varied across T1-T2 and T2-T3. These findings revealed the pattern and developmental characteristics of adolescents’ character strengths, as well as emphasized the influence of parental acceptance and rejection on the transitions of character strengths during early adolescence.</p>