In this paper, we study a fourth-order differential operator \(L_\lambda \) on a graph depending on a real parameter \(\lambda \) . The main question studied in the paper is determining the set of positive values of the spectral parameter \(\lambda \) for which the operator \(L_\lambda \) is positively invertible. We prove that \(L_\lambda \) for \(\lambda >0\) is positively invertible if and only if there is a fundamental system of solutions of the corresponding homogeneous equation consisting of functions that are positive on the graph. We formulate a necessary and sufficient condition for the differential operator \(L_\lambda \) to be positively invertible for all positive values of the spectral parameter less than the smallest eigenvalue of the differential operator \(L_0\) corresponding to the value \(\lambda =0\) . We establish the positivity of eigenvalues and prove a comparison theorem for eigenvalues of the spectral problem. We formulate maximum principles for fourth-order differential inequalities on the graph.