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<p><i>Background:</i> Diabetes often slows wound healing and can cause chronic ulcers. This study examined how Platelet-Rich Plasma (PRP), Low-Level Laser Therapy (LLLT), and their combination affect healing in diabetic rabbit wounds.</p><p><i>Methods:</i> Fourteen days after diabetes was induced, researchers created standardized wounds on the backs of 44 diabetic rabbits. The rabbits were randomly assigned to four groups: control (saline), PRP, LLLT, and PRP + LLLT, with 11&#xa0;rabbits per group and two wounds per rabbit. PRP was prepared just before use. LLLT was applied once daily for 14&#xa0;days using a&#xa0;non-contact method. Wound closure was assessed with digital photos on days&#xa0;7, 14, and&#xa0;28, and the percentage of fully closed wounds was recorded. Biomechanical tests were performed on days&#xa0;14 and&#xa0;28. Statistical analysis accounted for the two wounds per rabbit using Generalized Estimating Equations and Linear Mixed Models (LMM) with Tukey adjustment.</p><p><i>Results:</i> The proportion of closed wounds differed significantly among groups at day&#xa0;7 (p = 0.013), day&#xa0;14 (p = 0.001), and day&#xa0;28 (p = 0.001). At day&#xa0;28, the highest closure rate was observed in the PRP + LLLT group (86.3%), followed by LLLT (68.2%), PRP (54.5%), and control (22.7%). After adjustment for within-animal correlation, all interventions significantly improved wound closure compared to control; however, differences between LLLT and PRP alone were not statistically significant. Combination therapy demonstrated greater biomechanical strength than PRP alone, but its superiority over LLLT alone was not statistically significant for most outcomes.</p><p><i>Conclusion:</i> Both PRP and LLLT improved diabetic wound healing in this rabbit model. The combination of PRP + LLLT yielded the most favorable late-stage outcomes, especially compared to PRP alone, but its superiority over LLLT alone remains inconclusive. Proper statistical correction for clustering is essential in wound-healing studies involving multiple wounds per animal.</p>

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Effects of platelet-rich plasma and low-level laser therapy on cutaneous wound healing in a diabetic rabbit model

  • 期刊论文

  • 发表日期：

    2026年7月21日

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  • Ali TizMaghz,
  • Shahrokh Shojaei,
  • Pedram Tehrani

摘要

Background: Diabetes often slows wound healing and can cause chronic ulcers. This study examined how Platelet-Rich Plasma (PRP), Low-Level Laser Therapy (LLLT), and their combination affect healing in diabetic rabbit wounds.

Methods: Fourteen days after diabetes was induced, researchers created standardized wounds on the backs of 44 diabetic rabbits. The rabbits were randomly assigned to four groups: control (saline), PRP, LLLT, and PRP + LLLT, with 11 rabbits per group and two wounds per rabbit. PRP was prepared just before use. LLLT was applied once daily for 14 days using a non-contact method. Wound closure was assessed with digital photos on days 7, 14, and 28, and the percentage of fully closed wounds was recorded. Biomechanical tests were performed on days 14 and 28. Statistical analysis accounted for the two wounds per rabbit using Generalized Estimating Equations and Linear Mixed Models (LMM) with Tukey adjustment.

Results: The proportion of closed wounds differed significantly among groups at day 7 (p = 0.013), day 14 (p = 0.001), and day 28 (p = 0.001). At day 28, the highest closure rate was observed in the PRP + LLLT group (86.3%), followed by LLLT (68.2%), PRP (54.5%), and control (22.7%). After adjustment for within-animal correlation, all interventions significantly improved wound closure compared to control; however, differences between LLLT and PRP alone were not statistically significant. Combination therapy demonstrated greater biomechanical strength than PRP alone, but its superiority over LLLT alone was not statistically significant for most outcomes.

Conclusion: Both PRP and LLLT improved diabetic wound healing in this rabbit model. The combination of PRP + LLLT yielded the most favorable late-stage outcomes, especially compared to PRP alone, but its superiority over LLLT alone remains inconclusive. Proper statistical correction for clustering is essential in wound-healing studies involving multiple wounds per animal.