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<p>Sedimentary DNA (sedDNA) offers potential advantages for paleolimnological reconstructions by enabling better characterization of short- and long-term trends in aquatic communities. However, the temporal range and influence of different environmental variables on species-specific sedDNA distribution patterns are poorly understood, particularly for migratory species like diadromous fishes. Given their transient nature, it is unclear if and where diadromous fish sedDNA will accumulate in lake sediments, as well as to what extent sedDNA can capture historic diadromous fish signals. Using 12S rRNA metabarcoding, quantitative PCR, and occupancy modeling, we investigate the spatiotemporal distribution of fish assemblages and diadromous alewife (<i>Alosa pseudoharengus</i>) in sedDNA and eDNA samples from a coastal, oligotrophic lake in Maine, USA. Through paired water and sediment samples collected across various depths, habitats, and seasons, we found a heterogeneous distribution in fish sedDNA, with higher alewife detection probabilities associated with greater lake depth. While historic alewife sedDNA was detected in several samples, we highlight the potential rapid degradation and/or dilution rate of fish surface sedDNA in natural systems, contrasting previous studies. Collectively, our findings demonstrate that despite their seasonal presence, sediment focusing mechanisms play a critical role in the distribution and detection of diadromous alewife sedDNA, emphasizing lake depocenters as optimal locations for contemporary monitoring and historic reconstructions of these migratory species.</p>

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Assessing within-lake variation in migratory and resident fish aquatic and sedimentary DNA

  • 期刊论文

  • 发表日期：

    2026年5月22日

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  • Grayson P. Huston,
  • Jasmine E. Saros,
  • Michael T. Kinnison

摘要

Sedimentary DNA (sedDNA) offers potential advantages for paleolimnological reconstructions by enabling better characterization of short- and long-term trends in aquatic communities. However, the temporal range and influence of different environmental variables on species-specific sedDNA distribution patterns are poorly understood, particularly for migratory species like diadromous fishes. Given their transient nature, it is unclear if and where diadromous fish sedDNA will accumulate in lake sediments, as well as to what extent sedDNA can capture historic diadromous fish signals. Using 12S rRNA metabarcoding, quantitative PCR, and occupancy modeling, we investigate the spatiotemporal distribution of fish assemblages and diadromous alewife (Alosa pseudoharengus) in sedDNA and eDNA samples from a coastal, oligotrophic lake in Maine, USA. Through paired water and sediment samples collected across various depths, habitats, and seasons, we found a heterogeneous distribution in fish sedDNA, with higher alewife detection probabilities associated with greater lake depth. While historic alewife sedDNA was detected in several samples, we highlight the potential rapid degradation and/or dilution rate of fish surface sedDNA in natural systems, contrasting previous studies. Collectively, our findings demonstrate that despite their seasonal presence, sediment focusing mechanisms play a critical role in the distribution and detection of diadromous alewife sedDNA, emphasizing lake depocenters as optimal locations for contemporary monitoring and historic reconstructions of these migratory species.