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<p>This study investigated the valorization of spent coffee grounds (SCG) oil for polyhydroxyalkanoates (PHA) production using <i>Pseudomonas putida</i> KT2440 with an optimized detoxification pre-treatment. The detoxification process using ethanol and oil extraction using n-hexane significantly improved both <i>P. putida</i> growth and PHA accumulation, yielding up to 45.6% PHA from dried cell weight (DCW) as compared to 31.9% in the control without SCG pre-treatment. Optimized extraction process produced SCG oil rich in saturated fatty acids. The resulting polymer was a mixed short-chain-length (scl) and medium-chain-length (mcl)-PHA copolymer with 3-hydroxybutyrate and 3-hydroxyoctanoate as dominant monomers. A multiple regression model identified residual oil content as the primary factor affecting PHA content (% DCW), suggesting that pre-treated SCG oil is an ideal substrate for <i>P. putida</i> to accumulate mcl-PHA. To our knowledge, these results demonstrate, for the first time, the feasibility and efficacy of using detoxified SCG oil as a substrate for scl-mcl PHA copolymer production by <i>P. putida</i> KT2440, and highlight the critical role of detoxification as a process control variable for yield improvement.</p> Graphical Abstract <p></p>

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Valorization of Detoxified Spent Coffee Ground Oil for Enhanced Polyhydroxyalkanoate Biosynthesis by Pseudomonas putida KT2440

  • 期刊论文

  • 发表日期：

    2026年6月16日

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  • Aghasa Aghasa,
  • Sudharshan Juntupally,
  • Juyeon Yoon,
  • Eunseok Lee,
  • Hyung-Sool Lee

摘要

This study investigated the valorization of spent coffee grounds (SCG) oil for polyhydroxyalkanoates (PHA) production using Pseudomonas putida KT2440 with an optimized detoxification pre-treatment. The detoxification process using ethanol and oil extraction using n-hexane significantly improved both P. putida growth and PHA accumulation, yielding up to 45.6% PHA from dried cell weight (DCW) as compared to 31.9% in the control without SCG pre-treatment. Optimized extraction process produced SCG oil rich in saturated fatty acids. The resulting polymer was a mixed short-chain-length (scl) and medium-chain-length (mcl)-PHA copolymer with 3-hydroxybutyrate and 3-hydroxyoctanoate as dominant monomers. A multiple regression model identified residual oil content as the primary factor affecting PHA content (% DCW), suggesting that pre-treated SCG oil is an ideal substrate for P. putida to accumulate mcl-PHA. To our knowledge, these results demonstrate, for the first time, the feasibility and efficacy of using detoxified SCG oil as a substrate for scl-mcl PHA copolymer production by P. putida KT2440, and highlight the critical role of detoxification as a process control variable for yield improvement.

Graphical Abstract