Scholarlink

<p>We developed a bilayer dermal–epidermal skin substitute that structurally and functionally mimics native skin and supports the treatment of full-thickness wounds. The construct was incorporated with bone marrow mesenchymal stem cell (BM-MSC)–derived exosomes loaded with basic fibroblast growth factor (bFGF) to enhance and expedite the wound healing process. The epidermal-mimicking upper layer consisted of electrospun gelatin nanofibers, while the dermal-mimicking lower layer comprised an alginate hydrogel. Both gelatin and alginate are biocompatible polymers routinely used in commercially available wound dressings. Fourier transform infrared spectroscopy (FTIR) confirmed stable integration of the two layers through intermolecular interactions, including hydrogen bonding and electrostatic forces. The bFGF-loaded exosome-encapsulating scaffold (bFGF-Exo@A4G20; 4% alginate and 20% gelatin) exhibited several favorable properties essential for effective wound healing, including excellent biocompatibility with negligible cytotoxicity; suitable porosity (90.8%); an optimal swelling ratio (666.3%); efficient moisture retention; and the ability to support cell adhesion, proliferation, and migration. In line with emerging wound healing strategies that emphasize temporally coordinated therapeutic interventions, the alginate hydrogel layer enabled controlled and sustained release of bFGF-loaded exosomes, and provided prolonged bioactive signaling during critical proliferative and remodeling phases of tissue repair. In vivo, wounds treated with the bilayer construct showed significantly accelerated closure compared with controls, accompanied by enhanced re-epithelialization, increased epidermal thickness, and improved regeneration of skin appendages such as hair follicles and sebaceous glands. The treated wounds exhibited higher collagen deposition and more robust angiogenesis, which highlights the effectiveness of sustained pro-angiogenic signaling in functional tissue regeneration.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Fabrication and Evaluation of a Bilayer Gelatin Nanofiber/Alginate Hydrogel Skin Substitute with Sustained Release of bFGF-Loaded Mesenchymal Stem Cell-Derived Exosomes at the Wound Site

  • 期刊论文

  • 发表日期：

    2026年4月17日

查看全文
  • Shaghayegh Shahsavan,
  • Akram Alizadeh,
  • Fazel Sahraneshin Samani,
  • Vajihe Taghdiri Nooshabadi,
  • Hamid Reza Sameni

摘要

We developed a bilayer dermal–epidermal skin substitute that structurally and functionally mimics native skin and supports the treatment of full-thickness wounds. The construct was incorporated with bone marrow mesenchymal stem cell (BM-MSC)–derived exosomes loaded with basic fibroblast growth factor (bFGF) to enhance and expedite the wound healing process. The epidermal-mimicking upper layer consisted of electrospun gelatin nanofibers, while the dermal-mimicking lower layer comprised an alginate hydrogel. Both gelatin and alginate are biocompatible polymers routinely used in commercially available wound dressings. Fourier transform infrared spectroscopy (FTIR) confirmed stable integration of the two layers through intermolecular interactions, including hydrogen bonding and electrostatic forces. The bFGF-loaded exosome-encapsulating scaffold (bFGF-Exo@A4G20; 4% alginate and 20% gelatin) exhibited several favorable properties essential for effective wound healing, including excellent biocompatibility with negligible cytotoxicity; suitable porosity (90.8%); an optimal swelling ratio (666.3%); efficient moisture retention; and the ability to support cell adhesion, proliferation, and migration. In line with emerging wound healing strategies that emphasize temporally coordinated therapeutic interventions, the alginate hydrogel layer enabled controlled and sustained release of bFGF-loaded exosomes, and provided prolonged bioactive signaling during critical proliferative and remodeling phases of tissue repair. In vivo, wounds treated with the bilayer construct showed significantly accelerated closure compared with controls, accompanied by enhanced re-epithelialization, increased epidermal thickness, and improved regeneration of skin appendages such as hair follicles and sebaceous glands. The treated wounds exhibited higher collagen deposition and more robust angiogenesis, which highlights the effectiveness of sustained pro-angiogenic signaling in functional tissue regeneration.