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<p>The synthesis of a tropoelastin-based polypeptide (PP), poly(AAGVP) (where A denotes Alanine, G for Glycine, V for Valine, and P for Proline), is performed using a solution-phase approach. The miscibility of PP and polyvinyl alcohol (PVA) is examined in various ratios to develop membranes for suitable biomedical applications. Miscibility in the solution phase was evaluated using viscometric parameters (K<sub>H</sub>, ∆[η]<sub>m</sub>, ∆B, μ, α, β, ΔK as proposed by Huggins, Garcia, Chee, Sun, Jiang, and Han, individually), indicating that PVA is miscible with PP up to 60% of PP at 25&#xa0;°C. Viscosity increased with polymer concentration, being higher in PVA-rich systems but decreasing with increasing PP content. Intermolecular interactions between PP and PVA were confirmed by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and atomic force microscopy (AFM). Thermal stability of the blends, assessed by thermogravimetric analysis (TGA), showed improvements compared to pure PP. In vitro assays demonstrated α-amylase inhibition with IC<sub>50</sub> values of 0.81–0.85&#xa0;µg, outperforming the standard inhibitor acarbose (1.22&#xa0;µg). Enzyme kinetics (K<sub>m</sub> and V<sub>max</sub>) determined from Michaelis–Menten and Lineweaver–Burk plots confirmed this activity. These findings suggest that PP–PVA blends may be suitable candidates for biomedical applications.</p>

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Synthesis of Polypentapeptide of Tropoelastin and Exploration of Its Blends with Polyvinyl Alcohol for the Inhibition of α-Amylase and α-Glucosidase

  • 期刊论文

  • 发表日期：

    2026年1月12日

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  • H. R. Lokesh,
  • B. Mahesh,
  • Sangesh P. Zodape,
  • N. D. Rekha,
  • D. Channe Gowda,
  • Alina Sionkowska

摘要

The synthesis of a tropoelastin-based polypeptide (PP), poly(AAGVP) (where A denotes Alanine, G for Glycine, V for Valine, and P for Proline), is performed using a solution-phase approach. The miscibility of PP and polyvinyl alcohol (PVA) is examined in various ratios to develop membranes for suitable biomedical applications. Miscibility in the solution phase was evaluated using viscometric parameters (KH, ∆[η]m, ∆B, μ, α, β, ΔK as proposed by Huggins, Garcia, Chee, Sun, Jiang, and Han, individually), indicating that PVA is miscible with PP up to 60% of PP at 25 °C. Viscosity increased with polymer concentration, being higher in PVA-rich systems but decreasing with increasing PP content. Intermolecular interactions between PP and PVA were confirmed by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and atomic force microscopy (AFM). Thermal stability of the blends, assessed by thermogravimetric analysis (TGA), showed improvements compared to pure PP. In vitro assays demonstrated α-amylase inhibition with IC50 values of 0.81–0.85 µg, outperforming the standard inhibitor acarbose (1.22 µg). Enzyme kinetics (Km and Vmax) determined from Michaelis–Menten and Lineweaver–Burk plots confirmed this activity. These findings suggest that PP–PVA blends may be suitable candidates for biomedical applications.