<p>Treatment of wound infections has been a matter of concern due to the emergence of antimicrobial resistance (AMR) in <i>Pseudomonas aeruginosa</i> (PA). As an alternative to antibiotics, phage therapy has proven to be an effective strategy to combat AMR. The present study aimed to fabricate PA phage (PAΦ)-gentamicin (GeN)-loaded dressing made from chitosan and alginate, to thwart wound infections associated with PA. Various chitosan-alginate (CA) scaffolds (1&#xa0;C:1&#xa0;A/1&#xa0;C:3&#xa0;A/3&#xa0;C:1&#xa0;A) were prepared by physical crosslinking and subjected to parametric analysis for screening, followed by functionalizing using PAΦ-GeN combination. The findings revealed that 1&#xa0;C:1&#xa0;A dressing exhibited high swelling index (1887%), protein adsorption ability (845.8&#xa0;µg.cm^− 2), and in vitro biodegradability within 7 days. FTIR spectroscopy and thermogravimetric analysis supported biophysical characterization and electron microscopy revealed microporous structure of the dressing. Functionalized CA dressings demonstrated broad-range antibacterial potential against standard strains of <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Acinetobacter baumannii</i>, <i>Klebsiella pneumoniae</i>, and clinical strains of PA, while displaying a biocompatible/non-allergenic nature. The therapeutic potential of functionalized CA dressing was further validated in vivo using a thermal injury murine model infected with PA. Topical application of PAΦ-GeN-loaded CA dressing significantly lowered bacterial burden on day 3 post-infection (p.i.) and completely eradicated pseudomonal infection by day 21 p.i. Furthermore, the functionalized CA dressing significantly enhanced skin re-epithelialization and resulted in complete wound closure in BALB/c mice on day 21 p.i. Overall, the present study reinforces the application of functionalized wound dressings as promising alternative to traditional wound care products for management of skin infections.</p> Graphical Abstract <p></p>