Scholarlink

<p>In situ forming hydrogel scaffolds are essential in bone tissue engineering due to their ability to influence the behavior of seeded cells through their composition and physicochemical properties. However, many existing in situ forming hydrogels that rely solely on physical cross-linkers suffer from poor mechanical strength and inadequate gelation times. In this study, an interpenetrating network (IPN) hydrogel composed of silk fibroin (FIB), sodium alginate (ALG), and sodium alendronate (ALN) was developed, using Ca²⁺ ions as the physical cross-linking agent. ALN was incorporated into the FIB/ALG hydrogel matrix to enhance mechanical performance and regulate gelation kinetics. Increasing ALN content significantly improved the compressive modulus of the hydrogels from 28&#xa0;kPa to 67&#xa0;kPa. Additionally, the FIB/ALG/ALN hydrogels exhibited prolonged gelation times (25–72&#xa0;s), in contrast to the nearly instantaneous gelation observed in FIB/ALG hydrogels. In vitro release studies revealed a sustained release of ALN over 20 days, attributed to ionic interactions between the phosphate groups of ALN and the hydrogel network. Cell viability assays demonstrated excellent biocompatibility and confirmed the hydrogel’s ability to support stem cell adhesion, growth, and proliferation. These findings highlight the potential of FIB/ALG/ALN hydrogels as promising in situ forming scaffolds for bone tissue regeneration.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

In Vitro Characterization of Alginate/Silk Fibroin/Alendronate Hydrogels with Enhanced Mechanical Strength and Sustained Drug Release

  • 期刊论文

  • 发表日期：

    2026年1月27日

查看全文
  • Mahya Baradaran,
  • Fariba Ganji,
  • Hossein Shaki,
  • Habib Nikookar

摘要

In situ forming hydrogel scaffolds are essential in bone tissue engineering due to their ability to influence the behavior of seeded cells through their composition and physicochemical properties. However, many existing in situ forming hydrogels that rely solely on physical cross-linkers suffer from poor mechanical strength and inadequate gelation times. In this study, an interpenetrating network (IPN) hydrogel composed of silk fibroin (FIB), sodium alginate (ALG), and sodium alendronate (ALN) was developed, using Ca²⁺ ions as the physical cross-linking agent. ALN was incorporated into the FIB/ALG hydrogel matrix to enhance mechanical performance and regulate gelation kinetics. Increasing ALN content significantly improved the compressive modulus of the hydrogels from 28 kPa to 67 kPa. Additionally, the FIB/ALG/ALN hydrogels exhibited prolonged gelation times (25–72 s), in contrast to the nearly instantaneous gelation observed in FIB/ALG hydrogels. In vitro release studies revealed a sustained release of ALN over 20 days, attributed to ionic interactions between the phosphate groups of ALN and the hydrogel network. Cell viability assays demonstrated excellent biocompatibility and confirmed the hydrogel’s ability to support stem cell adhesion, growth, and proliferation. These findings highlight the potential of FIB/ALG/ALN hydrogels as promising in situ forming scaffolds for bone tissue regeneration.

Graphical Abstract