Scholarlink

<p>Chronic wounds and their associated infections pose a considerable challenge in clinical practice, often resulting in prolonged healing and increased healthcare costs. Berberine (BBR), a bioactive plant-derived alkaloid, exhibits a broad spectrum of pharmacological activities, including antimicrobial and anti-inflammatory effects. This study aimed to develop a bacterial nanocellulose (BNC) scaffold incorporated with BBR to systematically evaluate its antimicrobial efficacy and wound healing potential. The BNC-BBR composite scaffold was fabricated via a conventional freeze-drying method and thoroughly characterized using fourier infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). Antimicrobial activity was quantitatively assessed through colony count assays against key pathogens. An in vivo wound healing model involving a standardized 1 × 1&#xa0;cm full-thickness cutaneous wound on the dorsum of rats was employed, with scaffold application sustained over a 21-day period. Antimicrobial activity was assessed through colony count assays. The 1 × 1&#xa0;cm cutaneous wound model was created on the back of rats and the scaffolds were applied over the wounds, for 21 days. SEM analysis confirmed a homogeneous and porous scaffold architecture. The BNC-BBR scaffold demonstrated potent antimicrobial activity, achieving inhibition rates of 99.97% against <i>Escherichia coli</i> and 100% against <i>Candida albicans</i>. Histological analysis revealed that the BNC-BBR scaffold significantly promoted angiogenesis and fibroblast proliferation compared to the injured untreated animals (negative control) on day 7 post-wounding. Besides, the BNC-BBR scaffold significantly reduced inflammation on day 7 compared to the injured untreated animals. These findings indicate that the BNC scaffold loaded with Berberine accelerates cutaneous wound healing and exhibits strong antibacterial properties under in vitro conditions. This multifunctional scaffold thus represents a promising candidate for the development of advanced wound dressings with enhanced therapeutic efficacy.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of a Three-dimensional Bacterial Nano Cellulose Scaffold Concomitant with Berberine as a Therapeutic Modality in Wound Healing

  • 期刊论文

  • 发表日期：

    2025年9月25日

查看全文
  • Alireza Ghasempour,
  • Mahsa Barakchi,
  • Arefeh Jenaghi,
  • Effat Alemzadeh,
  • Ahmad Oryan,
  • Mahdieh Rajabi-Moghaddam,
  • Pouria Mohammadparast Tabas,
  • Esmat Alemzadeh,
  • Soroush Mohammadi

摘要

Chronic wounds and their associated infections pose a considerable challenge in clinical practice, often resulting in prolonged healing and increased healthcare costs. Berberine (BBR), a bioactive plant-derived alkaloid, exhibits a broad spectrum of pharmacological activities, including antimicrobial and anti-inflammatory effects. This study aimed to develop a bacterial nanocellulose (BNC) scaffold incorporated with BBR to systematically evaluate its antimicrobial efficacy and wound healing potential. The BNC-BBR composite scaffold was fabricated via a conventional freeze-drying method and thoroughly characterized using fourier infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). Antimicrobial activity was quantitatively assessed through colony count assays against key pathogens. An in vivo wound healing model involving a standardized 1 × 1 cm full-thickness cutaneous wound on the dorsum of rats was employed, with scaffold application sustained over a 21-day period. Antimicrobial activity was assessed through colony count assays. The 1 × 1 cm cutaneous wound model was created on the back of rats and the scaffolds were applied over the wounds, for 21 days. SEM analysis confirmed a homogeneous and porous scaffold architecture. The BNC-BBR scaffold demonstrated potent antimicrobial activity, achieving inhibition rates of 99.97% against Escherichia coli and 100% against Candida albicans. Histological analysis revealed that the BNC-BBR scaffold significantly promoted angiogenesis and fibroblast proliferation compared to the injured untreated animals (negative control) on day 7 post-wounding. Besides, the BNC-BBR scaffold significantly reduced inflammation on day 7 compared to the injured untreated animals. These findings indicate that the BNC scaffold loaded with Berberine accelerates cutaneous wound healing and exhibits strong antibacterial properties under in vitro conditions. This multifunctional scaffold thus represents a promising candidate for the development of advanced wound dressings with enhanced therapeutic efficacy.

Graphical Abstract