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<p>The present work is devoted to the search for new bacterial species capable for hydrolysis of industrial polymeric materials containing aromatic monomers such as: polyethylene terephthalate (PET), polystyrene (PS), and polyurethane (PUR). Methods for obtaining agar media with PET-, PS-, and PUR-nanoparticles were developed for bacterial screening. As a result, new species of <i>Streptomyces</i> hydrolyzing all three types of plastic were found. The selected bacteria were capable for production of various hydrolases with different substrate selectivity towards polymeric compounds such as: olive oil, Tween 20, Tween 80, polycaprolactone (PCL), PET, PS, and PUR. At the same time, enzymes that hydrolyzed industrial plastics differed in induction time and substrate selectivity from the other hydrolases produced by the same bacteria. It was shown that only Tween 80 from all tested polymers of natural and artificial origin increased the hydrolase activity of bacteria towards PET-, PS-, and PUR-nanoparticles during solid-state cultivation. Universal primers have been developed based on all known genes encoding enzymes with PETase-like activity. The primers have been used to demonstrate the presence of PETase-like genes in the genomes of the selected streptomycetes. Phylogenetic analysis of the obtained nucleotide sequences confirms that they belong to the genes encoding enzymes with PETase activity. The presence of PETase-like hydrolase genes in the genomes of the new streptomycetes, along with the wide distribution of streptomycetes in soil, water resources, on plants and animals makes them a promising source of new hydrolases degrading industrial plastic.</p>

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Novel Soil Bacteria of the Genus Streptomyces Hydrolyzing Industrial Aromatic Polymeric Compounds

  • 期刊论文

  • 发表日期：

    2025年9月23日

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  • M.P. Kolomytseva,
  • A.M. Chernykh,
  • A.D. Egorova,
  • S.Yu. Gorina,
  • K.A. Shestakova,
  • Zh.V. Renfeld,
  • A.V. Sybachin,
  • A.S. Gaidina,
  • S.S. Karlov,
  • N.A. Belova,
  • O.V. Moiseeva

摘要

The present work is devoted to the search for new bacterial species capable for hydrolysis of industrial polymeric materials containing aromatic monomers such as: polyethylene terephthalate (PET), polystyrene (PS), and polyurethane (PUR). Methods for obtaining agar media with PET-, PS-, and PUR-nanoparticles were developed for bacterial screening. As a result, new species of Streptomyces hydrolyzing all three types of plastic were found. The selected bacteria were capable for production of various hydrolases with different substrate selectivity towards polymeric compounds such as: olive oil, Tween 20, Tween 80, polycaprolactone (PCL), PET, PS, and PUR. At the same time, enzymes that hydrolyzed industrial plastics differed in induction time and substrate selectivity from the other hydrolases produced by the same bacteria. It was shown that only Tween 80 from all tested polymers of natural and artificial origin increased the hydrolase activity of bacteria towards PET-, PS-, and PUR-nanoparticles during solid-state cultivation. Universal primers have been developed based on all known genes encoding enzymes with PETase-like activity. The primers have been used to demonstrate the presence of PETase-like genes in the genomes of the selected streptomycetes. Phylogenetic analysis of the obtained nucleotide sequences confirms that they belong to the genes encoding enzymes with PETase activity. The presence of PETase-like hydrolase genes in the genomes of the new streptomycetes, along with the wide distribution of streptomycetes in soil, water resources, on plants and animals makes them a promising source of new hydrolases degrading industrial plastic.