<p>Quorum Quenching (QQ) is a proven anti-biofouling strategy in Membrane Bioreactors (MBRs). This study presents a novel approach for preparing and characterizing Poly (vinyl alcohol) (PVA) and Alginate (ALG) sheets for biofouling mitigation in MBRs. A significant challenge is posed in achieving QQ sheet preparation without using boric acid (H₃BO₃), a toxic crosslinker, and in a single-step crosslinking process. In this context, novel PVA-ALG sheets were prepared using sodium nitrate (NaNO₃) and calcium chloride (CaCl₂) in a single crosslinking step. Another new type of porous sheets was also prepared using a primary crosslinking solution composed of H₃BO₃, NaNO₃, and CaCl₂. The structural, mechanical, and textural properties of newly prepared sheets were compared to those of sheets crosslinked using the conventional primary crosslinking solution containing H₃BO₃ and CaCl₂. Additionally, degradation of the QS signal molecule C8-HSL was investigated using all types of sheets entrapped with the QQ bacteria <i>Rhodococcus</i> sp. BH4. Differences in the composition of the crosslinking solutions among the various sheet types led to variations in their structural properties, characterized by increased crosslinking density and macro-void formation. Mesopores dominate in all PVA-ALG sheets, while their specific surface areas range from 45 m²/g to 77 m²/g, and tensile strength between 0.1&#xa0;MPa and 0.3&#xa0;MPa. New sheets crosslinked with NaNO₃ and CaCl₂ display the highest tensile strength and specific surface area. Furthermore, QQ sheets crosslinked with NaNO₃ and CaCl₂ demonstrated a superior C8-HSL degradation capacity compared to those prepared using the conventional crosslinking method involving boric acid.</p> Graphical Abstract <p></p>