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<p>A porous crystalline material known as metal-organic frameworks (MOFs), consisting of metal ions and organic linkers, exhibits a high surface area and adjustable porosity, making it suitable for applications such as drug delivery, gas storage, and catalysis. In this study, we have utilized gallic acid, a plant phenolic acid, as a reducing agent to synthesize silver nanoparticles, which are then incorporated within the OX-2 MOF which itself is silver-based. UV-visible spectroscopy, FTIR, XRD, Zeta potential, FESEM, EDAX, HRTEM, and SAED analysis were employed to determine the size, shape, stability, surface morphology and crystallinity of synthesized nanostructures. Inductively coupled plasma mass spectroscopy (ICP-MS) analysis was employed to study the successful incorporation of silver nanoparticles into the OX-2 MOF. The cytocompatibility of the nanocomposites was established using the MTT assay in normal mouse fibroblast L929 cells, whereas the nanocomposites induced significant cytotoxicity in SKOV-3 ovarian cancer cells. Apoptotic potential of the Ag@OX-2 nanocomposites was further evaluated using AO/EtBr double staining and Annexin V/Propidium Iodide apoptosis assay. It was observed that 47.60% of SKOV 3 cells underwent apoptosis after the nanocomposite treatment, confirming apoptosis as the prominent mechanism of cancer cell death. ROS mediated DNA damage apoptosis induced Ag@OX-2 nanocomposites were further validated through ROS generation study and Comet assay.</p>

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Synthesis, characterization, cytocompatibility study and anticancer effect of OX-2 Metal Organic Framework/Silver nanocomposites

  • 期刊论文

  • 发表日期：

    2026年6月19日

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  • K. S. Dhanya,
  • M. Anaswara Biju,
  • U. Arun,
  • R. Sreeja

摘要

A porous crystalline material known as metal-organic frameworks (MOFs), consisting of metal ions and organic linkers, exhibits a high surface area and adjustable porosity, making it suitable for applications such as drug delivery, gas storage, and catalysis. In this study, we have utilized gallic acid, a plant phenolic acid, as a reducing agent to synthesize silver nanoparticles, which are then incorporated within the OX-2 MOF which itself is silver-based. UV-visible spectroscopy, FTIR, XRD, Zeta potential, FESEM, EDAX, HRTEM, and SAED analysis were employed to determine the size, shape, stability, surface morphology and crystallinity of synthesized nanostructures. Inductively coupled plasma mass spectroscopy (ICP-MS) analysis was employed to study the successful incorporation of silver nanoparticles into the OX-2 MOF. The cytocompatibility of the nanocomposites was established using the MTT assay in normal mouse fibroblast L929 cells, whereas the nanocomposites induced significant cytotoxicity in SKOV-3 ovarian cancer cells. Apoptotic potential of the Ag@OX-2 nanocomposites was further evaluated using AO/EtBr double staining and Annexin V/Propidium Iodide apoptosis assay. It was observed that 47.60% of SKOV 3 cells underwent apoptosis after the nanocomposite treatment, confirming apoptosis as the prominent mechanism of cancer cell death. ROS mediated DNA damage apoptosis induced Ag@OX-2 nanocomposites were further validated through ROS generation study and Comet assay.