Scholarlink

<p>This study reports a green and sustainable synthesis of a graphene oxide/vanadium pentoxide (GO/V<sub>2</sub>O<sub>5</sub>) nanocomposite using an ethanolic leaf extract of&#xa0;<i>Eichhornia crassipes</i>&#xa0;(water hyacinth), an invasive aquatic plant rich in bioactive phytochemicals. The presence of alkaloids, polyphenols, and flavonoids in the extract facilitating the nanocomposite reduction and stabilization. The structural and compositional studies to XRD, EDX and FT-IR conforming the crystals formation, also the composition of the formation of crystals, the composition of elements, and the functional groups involved in the coating, beside the results of FESEM conducted the nanosheets shape with sizes ranging from 39–50 nm. The biologically synthesized GO/V<sub>2</sub>O<sub>5</sub> nanoparticles reflect a strong effective results with concentration –dependent bactericidal activity, with more than 90% inhibition at 60 µg.mL<sup>−1</sup> against <i>Streptococcus pneumoniae</i> (MIC 60 µg.mL<sup>−1</sup>, MBC 80 µg.mL<sup>−1</sup>) and <i>Klebsiella pneumoniae</i> with (MBC 100 µg.mL<sup>−1</sup>),surpassing the crude extract. The evaluation of antioxidant using DPPH investigated the noticeable activity to the extract as free radical scavenging agent compared to ascorbic acid. Furthermore, the antibiofilm activity observed a significant effect with more than 97% and 95% inhibition to biofilm formation at 100 µg.mL<sup>−1</sup> for <i>S. pneumoniae</i> and <i>K. pneumoniae</i>, separately. To elucidate the molecular mechanism underlying this anti-biofilm efficacy,&#xa0;in silico&#xa0;molecular docking was conducted. The main surface-capping phytochemicals (phytol, oleic acid, and octadecatrienoic acid) demonstrated a high-affinity binding against the master quorum-sensing targets SdiA and LuxS, outperforming standard antibiotics in neutralizing intercellular signaling. This work highlights the valorization of&#xa0;<i>E. crassipes</i>&#xa0;into multifunctional nanomaterials where the physical damage from the nanocomposite interface and targeted molecular quorum-sensing inhibition synergistically combats resistant pathogens, strongly supporting both green nanotechnology and ecological sustainability.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Green Synthesized Vanadium Pentoxide@Graphene Oxide Nanocomposite as a Broad-Spectrum Antibacterial Nanomaterial: Integrated In Vitro and In Silico Investigations

  • 期刊论文

  • 发表日期：

    2026年5月22日

查看全文
  • Saba A. Mahdy,
  • Maryam Al Ameri,
  • Ragheed Hussam Yousif,
  • Buthenia A. Hasoon,
  • Majid S. Jabir,
  • Suresh Ghotekar,
  • Dong Li,
  • Ayman A. Swelum

摘要

This study reports a green and sustainable synthesis of a graphene oxide/vanadium pentoxide (GO/V2O5) nanocomposite using an ethanolic leaf extract of Eichhornia crassipes (water hyacinth), an invasive aquatic plant rich in bioactive phytochemicals. The presence of alkaloids, polyphenols, and flavonoids in the extract facilitating the nanocomposite reduction and stabilization. The structural and compositional studies to XRD, EDX and FT-IR conforming the crystals formation, also the composition of the formation of crystals, the composition of elements, and the functional groups involved in the coating, beside the results of FESEM conducted the nanosheets shape with sizes ranging from 39–50 nm. The biologically synthesized GO/V2O5 nanoparticles reflect a strong effective results with concentration –dependent bactericidal activity, with more than 90% inhibition at 60 µg.mL−1 against Streptococcus pneumoniae (MIC 60 µg.mL−1, MBC 80 µg.mL−1) and Klebsiella pneumoniae with (MBC 100 µg.mL−1),surpassing the crude extract. The evaluation of antioxidant using DPPH investigated the noticeable activity to the extract as free radical scavenging agent compared to ascorbic acid. Furthermore, the antibiofilm activity observed a significant effect with more than 97% and 95% inhibition to biofilm formation at 100 µg.mL−1 for S. pneumoniae and K. pneumoniae, separately. To elucidate the molecular mechanism underlying this anti-biofilm efficacy, in silico molecular docking was conducted. The main surface-capping phytochemicals (phytol, oleic acid, and octadecatrienoic acid) demonstrated a high-affinity binding against the master quorum-sensing targets SdiA and LuxS, outperforming standard antibiotics in neutralizing intercellular signaling. This work highlights the valorization of E. crassipes into multifunctional nanomaterials where the physical damage from the nanocomposite interface and targeted molecular quorum-sensing inhibition synergistically combats resistant pathogens, strongly supporting both green nanotechnology and ecological sustainability.

Graphical Abstract