Abstract <p>Nanomedicine has generated significant interest in manipulating the tumor microenvironment for cancer therapy. This study introduces a novel ternary hybrid nanocomposite, Au@Pt-TiO₂, synthesized through a one-pot solvothermal process, exhibiting multifunctional properties as a sonosensitizer and multi-enzyme mimic for tumor therapy. This nanocomposite holds potential for sonodynamic therapy (SDT) due to its ability to generate reactive oxygen species (ROS) under ultrasound excitation. It addresses tumor hypoxia and glucose energy supply via catalase and glucose oxidase activities. Peroxidase mimetic activity also addresses this by generating ·OH radicals. This evaluation was performed in the presence of hydrogen peroxide (H₂O₂) and a colorimetric indicator (OPD), allowing the extraction of kinetic parameters using the Michaelis-Menten model. The nanocomposite’s synergistic effects arise from enhanced electron-hole separation, catalytic activities, and strong asymmetric electric coupling. Preliminary results suggest the nanocomposite’s promise for advancing cancer therapy, although further studies are needed to explore its biocompatibility and in vivo performance. This multifunctional approach offers an innovative strategy in the evolving field of nanomedicine for advanced therapeutic applications.</p> Graphical Abstract <p></p>