<p>As the global population ages, enhancing the mental health of older adults has become increasingly important. Meditation practices have shown promise in improving subjective well-being, an important component of mental health in later life, but their effectiveness in online interactive formats tailored to older adults remains underexplored. This study investigated the effect of an online interactive intervention that combines mindfulness meditation (MM) with loving-kindness and compassion meditation (LKCM) on older adults’ subjective well-being. A randomized controlled trial was conducted with 213 participants who were assigned to either a meditation intervention or an active control group. Measures of subjective well-being, meaning in life, mindfulness, and self-compassion were collected at pre-test, post-test, and one-month follow-up. Daily assessments of subjective well-being and meaning in life were also conducted throughout the intervention period. The meditation group demonstrated significant improvements in mindfulness, self-compassion, and subjective well-being from the pre-test to the post-test, with sustained enhancements in subjective well-being and self-compassion at the follow-up. Daily assessments indicated that participants in the meditation group reported higher daily subjective well-being across the intervention period compared with the control group, along with modest increases in daily meaning ratings. These findings support the effectiveness of our intervention, providing implications for the development of accessible and cost-effective subjective well-being focused online interventions to promote healthy aging.</p>