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<p>Viscosity and peroxynitrite (ONOO⁻) are critical parameters of the cellular microenvironment, and their abnormal fluctuations are closely associated with the progression of various diseases. Leveraging the twisted intramolecular charge transfer (TICT) mechanism, we have designed and synthesized a novel dual-function fluorescent probe (VMI). In this probe, a cyanopyridine moiety is linked to 6-methoxy-2-naphthalene through an unsaturated C = C bond that functions both as the TICT switch for viscosity sensing and as a specific reaction site for ONOO⁻. Under varying viscosity conditions, <b>VMI</b> modulates green fluorescence intensity to visualize changes in viscosity. Concurrently, ONOO⁻-mediated oxidative cleavage of the C = C bond releases a blue-emitting fluorophore, facilitating the monitoring of ONOO⁻ levels. Spectroscopic studies demonstrate that <b>VMI</b> exhibits high selectivity, excellent anti-interference capabilities, a low detection limit of 16.11 nΜ, rapid response time (within 10&#xa0;min), and minimal cytotoxicity. Live-cell imaging further validates its capacity to simultaneously track dynamic alterations in viscosity and ONOO⁻ levels within biological systems.</p>

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A TICT‐Activated Dual‐Function Fluorescent Probe for Imaging of Intracellular Viscosity and Peroxynitrite

  • 期刊论文

  • 发表日期：

    2026年1月21日

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  • Zhijie Zheng,
  • Ya-Long Zheng

摘要

Viscosity and peroxynitrite (ONOO⁻) are critical parameters of the cellular microenvironment, and their abnormal fluctuations are closely associated with the progression of various diseases. Leveraging the twisted intramolecular charge transfer (TICT) mechanism, we have designed and synthesized a novel dual-function fluorescent probe (VMI). In this probe, a cyanopyridine moiety is linked to 6-methoxy-2-naphthalene through an unsaturated C = C bond that functions both as the TICT switch for viscosity sensing and as a specific reaction site for ONOO⁻. Under varying viscosity conditions, VMI modulates green fluorescence intensity to visualize changes in viscosity. Concurrently, ONOO⁻-mediated oxidative cleavage of the C = C bond releases a blue-emitting fluorophore, facilitating the monitoring of ONOO⁻ levels. Spectroscopic studies demonstrate that VMI exhibits high selectivity, excellent anti-interference capabilities, a low detection limit of 16.11 nΜ, rapid response time (within 10 min), and minimal cytotoxicity. Live-cell imaging further validates its capacity to simultaneously track dynamic alterations in viscosity and ONOO⁻ levels within biological systems.