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<p>Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02–0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.</p>

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Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月20日

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  • Fatma Doğan Güzel,
  • Hamed Ghorbanpoor

摘要

Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02–0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.