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<p>Here we propose an eco-friendly hydrothermal approach to synthesise fluorescent L-cysteine capped zirconium oxide quantum dots (L-Cys-ZrO<sub>2</sub> QDs). The UV–<i>Vis</i> absorption, PL-emission, <i>pH</i> independence, size, functional group attached over surface QDs, binding energy, and stability of QDs in aqueous solvent were systematically studied. The TEM results revealed the mean particle size ∼5.7&#xa0;nm of QDs. The synthesised QDs have UV–<i>Vis</i> absorption peaks at 320&#xa0;nm, 265&#xa0;nm, and 245&#xa0;nm with PL emission from 360&#xa0;nm to 500&#xa0;nm and have a quantum yield ∼3.6%. The functional groups attached over surface of QDs such as –CO, -NH<sub>2</sub>, SO<sub>4</sub><sup>−−</sup> etc. were confirmed by FT-IR spectrum which were supported by XPS spectrum analysis. An in-vitro optical bio-imaging method was employed, focusing on cancer cell line such as HeLa Cells as a model cell. Cytotoxicity is analysed using MTT assay showing that QDs are biocompatible in nature. The photoluminescence properties of the synthesized L-Cys-ZrO<sub>2</sub> QDs were examined on the HeLa cells under green laser. The quantum dots proved to be effective labels for bioimaging as evidenced by strong emission when observed under a confocal fluorescence microscope by green laser.</p>

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Synthesis and Study of pH Independent L-Cysteine Capped Zirconium Oxide Quantum Dots as a Potential Bio-Imaging Probe Using HeLa Cells

  • 期刊论文

  • 发表日期：

    2025年3月4日

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  • Surya Pratap,
  • Himanshu Meena,
  • Shashi kant sharma,
  • Vijay Kumar Sonkar,
  • Sundeep Kumar,
  • Horesh Kumar

摘要

Here we propose an eco-friendly hydrothermal approach to synthesise fluorescent L-cysteine capped zirconium oxide quantum dots (L-Cys-ZrO2 QDs). The UV–Vis absorption, PL-emission, pH independence, size, functional group attached over surface QDs, binding energy, and stability of QDs in aqueous solvent were systematically studied. The TEM results revealed the mean particle size ∼5.7 nm of QDs. The synthesised QDs have UV–Vis absorption peaks at 320 nm, 265 nm, and 245 nm with PL emission from 360 nm to 500 nm and have a quantum yield ∼3.6%. The functional groups attached over surface of QDs such as –CO, -NH2, SO4−− etc. were confirmed by FT-IR spectrum which were supported by XPS spectrum analysis. An in-vitro optical bio-imaging method was employed, focusing on cancer cell line such as HeLa Cells as a model cell. Cytotoxicity is analysed using MTT assay showing that QDs are biocompatible in nature. The photoluminescence properties of the synthesized L-Cys-ZrO2 QDs were examined on the HeLa cells under green laser. The quantum dots proved to be effective labels for bioimaging as evidenced by strong emission when observed under a confocal fluorescence microscope by green laser.