Scholarlink

<p>Roughly spherical-shaped MnO₂ nanoparticles (NPs; 1–5.5&#xa0;nm), Ag/AgO/Ag₂O NPs (4–18&#xa0;nm), and Ag/Mn₃O₄ nanocomposites (6–24&#xa0;nm) were biosynthesised and evaluated for their antimicrobial activity against critical and high-priority bacterial pathogens listed in the Indian Priority-Pathogen List, along with common antibiotic-resistant waterborne bacteria. Antimicrobial efficacy was assessed using disc-diffusion and growth-curve assays against <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Bacillus cereus</i>, <i>Vibrio cholerae</i>, <i>Streptococcus pyogenes</i>, and <i>Proteus mirabilis</i>. MnO₂ NPs did not exhibit antimicrobial activity against any of the eight tested strains. In contrast, Ag/AgO/Ag₂O NPs inhibited six of the eight strains, showing a maximum zone of inhibition (ZOI) of 5.3 ± 0.6&#xa0;mm against <i>S. aureus</i>. The Ag/Mn₃O₄ nanocomposite demonstrated superior antimicrobial performance, inhibiting all eight bacterial strains with greater efficacy than Ag/AgO/Ag₂O NPs, and produced a maximum ZOI of 6.7 ± 0.6&#xa0;mm against <i>P. aeruginosa</i>. For <i>B. cereus</i>, both nanomaterials exhibited minimum inhibitory concentrations of 2 ng/µL and IC₅₀ values of 0.7 ng/µL; however, the minimum bactericidal concentration for Ag/AgO/Ag₂O NPs was 4 ng/µL. Haemocompatibility analysis revealed that the non-haemolytic concentrations for Ag/AgO/Ag₂O, MnO₂, and Ag/Mn₃O₄ nanomaterials were 0.01, 1.0, and 0.1&#xa0;µg/µL, respectively, while the haemolytic concentrations (HC₁₀) values for Ag/AgO/Ag₂O and Ag/Mn₃O₄ were 0.3 and 0.6&#xa0;µg/µL, respectively.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Solanum tuberosum-Based Biosynthesis of Ag/Mn3O4 Nanocomposite for Inhibition of Indian Critical and High-priority Pathogen Listed Bacteria

  • 期刊论文

  • 发表日期：

    2026年7月9日

查看全文
  • Bandita Panda,
  • Archita Lenka,
  • Sandip Kumar Dash,
  • Ganngam Phaomei,
  • Sukanta Kumar Tripathy

摘要

Roughly spherical-shaped MnO₂ nanoparticles (NPs; 1–5.5 nm), Ag/AgO/Ag₂O NPs (4–18 nm), and Ag/Mn₃O₄ nanocomposites (6–24 nm) were biosynthesised and evaluated for their antimicrobial activity against critical and high-priority bacterial pathogens listed in the Indian Priority-Pathogen List, along with common antibiotic-resistant waterborne bacteria. Antimicrobial efficacy was assessed using disc-diffusion and growth-curve assays against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Vibrio cholerae, Streptococcus pyogenes, and Proteus mirabilis. MnO₂ NPs did not exhibit antimicrobial activity against any of the eight tested strains. In contrast, Ag/AgO/Ag₂O NPs inhibited six of the eight strains, showing a maximum zone of inhibition (ZOI) of 5.3 ± 0.6 mm against S. aureus. The Ag/Mn₃O₄ nanocomposite demonstrated superior antimicrobial performance, inhibiting all eight bacterial strains with greater efficacy than Ag/AgO/Ag₂O NPs, and produced a maximum ZOI of 6.7 ± 0.6 mm against P. aeruginosa. For B. cereus, both nanomaterials exhibited minimum inhibitory concentrations of 2 ng/µL and IC₅₀ values of 0.7 ng/µL; however, the minimum bactericidal concentration for Ag/AgO/Ag₂O NPs was 4 ng/µL. Haemocompatibility analysis revealed that the non-haemolytic concentrations for Ag/AgO/Ag₂O, MnO₂, and Ag/Mn₃O₄ nanomaterials were 0.01, 1.0, and 0.1 µg/µL, respectively, while the haemolytic concentrations (HC₁₀) values for Ag/AgO/Ag₂O and Ag/Mn₃O₄ were 0.3 and 0.6 µg/µL, respectively.

Graphical Abstract