<p>Graphene-like structured molybdenum disulfide (MoS₂) is an indirect band gap material suitable as a supporting platform for developing nanosensors. The presence of abundant edge sites and the high surface area in its nanoflower morphology enhance its catalytic activity, making it a superior material for electrochemical sensing. The present work describes the use of silver nanoparticles decorated on flower-like structured molybdenum disulfide to improve charge-transfer properties and thereby increase the sensitivity toward acetaminophen. Here, MoS₂ is prepared by a conventional hydrothermal method, followed by chemical reduction of silver to produce Ag/MoS₂ nanostructures. The structural, optical, and morphological properties of the synthesized material were characterized to evaluate its potential for sensor applications. A carbon paste electrode was modified with Ag/MoS₂ and utilized for the electrochemical sensing of acetaminophen. Electrochemical techniques such as CV and EIS were employed to understand the effects of pH, active layer thickness, scan rate, and charge-transport properties across the electrodes. The differential pulse voltammetry (DPV) technique was used to study the effect of analyte concentration on anodic current and for the construction of the calibration plot. Interference, reproducibility, repeatability, and shelf-life were also analyzed. The electrochemical sensor exhibited an excellent operational range of 8 × 10⁻³ to 8 × 10⁻⁵ M with a limit of detection (LOD) of 4.35 µM. In comparison to other similar acetaminophen sensors, the present sensor offers the advantages of highly basic operating conditions, low cost, ease of fabrication, and surface renewability. Real-life analysis using commercially available APAP drug samples showed excellent recovery percentages.</p> Graphical Abstract <p></p>