<p>A colorimetric sensor was developed based on the mixture of hydrothermally synthesized tungsten oxide nanorods (WO₃ NRs) and copper-based metal-organic frameworks (Cu-MOFs) in order to detect hydrogen peroxide (H₂O₂) and glucose with high sensitivity and selectivity. By changing pH, temperature and Cu-MOFs: WO₃ NRs concentrations, the peroxidase-like enzymatic activity of Cu-MOFs: WO₃ NRs was investigated to find optimum conditions of the oxidization of 3,3’,5,5’ tetramethylbenzidine (TMB) in the presence of H₂O₂. Moreover, TMB oxidation steady-state kinetics studies are performed in the presence of Cu-MOFs: WO₃ NRs in order to obtain Michaelis-Menten constant value (K_m) as a kinetic parameter using Lineweaver-Burk plot. The K_m value of the sensor for H₂O₂ substrate (0.694 mM) is found to be less than that for horseradish peroxidase enzyme (3.7 mM), indicating its higher affinity towards H₂O₂ with a detection limit of 1.66 µM due to the effective intimate mixing of WO₃ NRs and Cu-MOFs. The proposed sensor is also used for colorimetric detection of sugar such as glucose, galactose, sucrose, lactose, fructose and ribose, resulting in a linear range up to 100 µM and low detection limit of 0.42 µM with short response time for glucose detection. The mixture of WO₃ NRs and Cu-MOFs is intended to synergistically enhance the catalytic activity by improving electron transfer efficiency and light absorption properties. Accordingly, Cu-MOFs: WO₃ NRs with low-cost synthesis and ease of modification exhibits good peroxidase-like activity, being suitable to serve as a high performance substitute for natural peroxidases in biosensors, medicines, catalysis and food industry applications. *Corresponding author at: Department of Physics, University of Kashan, Kashan, Iran. E-mail: zhdfr@kashanu.ac.ir ; Tel: +98 3,155,912,577 (M. Zahedifar).</p> Graphical Abstract <p></p>