Scholarlink

<p>Nanostructured materials with tailored morphologies exhibit unique physicochemical properties, making them highly attractive for catalysis, sensing, and biomedical applications. In this study, Copper/Cuprous oxide Yolk-Shell Nanostructures (Cu/Cu<sub>2</sub>O-YSN) were synthesized via an eco-friendly approach using <i>Cocos nucifera</i> inflorescence (CnI) extracts as a bio-reducing agent in Fehling’s solution. By tuning the redox environment following Le Chatelier’s principle, precise control over nucleation and growth was achieved, leading to well-defined yolk-shell architectures. Real-time UV-Vis spectral analysis monitored the evolution of Cu/Cu<sub>2</sub>O-YSN. Characterization techniques (UV-Vis, p-XRD, FE-SEM &amp; EDX, TEM, HR-TEM, and SAED) confirmed the biphasic architecture, comprising cubic Cu and Cu<sub>2</sub>O domains, with metallic copper constituting approximately 52% of the material. Yolk-shell formation was driven by interfacial mechanisms such as the nano-Kirkendall effect, Ostwald ripening, and Cabrera-Mott oxidation. A highly negative ζ potential of -48.38 mV indicated excellent colloidal stability due to phytochemical surface functionalization. UV-Vis spectral analysis revealed distinct isosbestic points at 304&#xa0;nm, 343&#xa0;nm, and 431&#xa0;nm, suggesting a two-step electron/proton transfer mechanism (DPPH<sup>•</sup> → DPPH⁻ → DPPH-H) during radical scavenging, with antioxidant activity (IC₅₀ = 40.58&#xa0;µg mL<sup>-1</sup>). The Cu/Cu<sub>2</sub>O-YSN also demonstrated broad spectrum antibacterial activity, showing high sensitivity toward Gram-positive strains and significant cytotoxicity against human liver cancer cells - HepG2 (IC<sub>50</sub> = 25 ± 0.5&#xa0;µg mL<sup>-1</sup>), comparable to doxorubicin. Apoptotic features including membrane blebbing, chromatin condensation, and nuclear fragmentation were confirmed by Acridine Orange (AO/EB) dual staining and 4′,6-diamidino-2-phenylindole (DAPI) imaging. These findings establish green synthesized Cu/Cu<sub>2</sub>O-YSN as promising multifunctional nanomaterials for advanced biomedical applications.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Sustainable Synthesis of Cu/Cu2O Yolk-Shell Nanostructures: Characterizations, DPPH• Interaction, Antibacterial Properties, and Cytotoxicity Against HepG2 Cells

  • 期刊论文

  • 发表日期：

    2026年1月13日

查看全文
  • Srinivasan Anbalagan,
  • Jayashanthini Sudalaimani,
  • Gayathri Gopalakrishnan,
  • Velmani Nanjan,
  • Rajagopal Gurusamy,
  • Rajasekar Krishnan,
  • Ramasubbu Alagunambi

摘要

Nanostructured materials with tailored morphologies exhibit unique physicochemical properties, making them highly attractive for catalysis, sensing, and biomedical applications. In this study, Copper/Cuprous oxide Yolk-Shell Nanostructures (Cu/Cu2O-YSN) were synthesized via an eco-friendly approach using Cocos nucifera inflorescence (CnI) extracts as a bio-reducing agent in Fehling’s solution. By tuning the redox environment following Le Chatelier’s principle, precise control over nucleation and growth was achieved, leading to well-defined yolk-shell architectures. Real-time UV-Vis spectral analysis monitored the evolution of Cu/Cu2O-YSN. Characterization techniques (UV-Vis, p-XRD, FE-SEM & EDX, TEM, HR-TEM, and SAED) confirmed the biphasic architecture, comprising cubic Cu and Cu2O domains, with metallic copper constituting approximately 52% of the material. Yolk-shell formation was driven by interfacial mechanisms such as the nano-Kirkendall effect, Ostwald ripening, and Cabrera-Mott oxidation. A highly negative ζ potential of -48.38 mV indicated excellent colloidal stability due to phytochemical surface functionalization. UV-Vis spectral analysis revealed distinct isosbestic points at 304 nm, 343 nm, and 431 nm, suggesting a two-step electron/proton transfer mechanism (DPPH• → DPPH⁻ → DPPH-H) during radical scavenging, with antioxidant activity (IC₅₀ = 40.58 µg mL-1). The Cu/Cu2O-YSN also demonstrated broad spectrum antibacterial activity, showing high sensitivity toward Gram-positive strains and significant cytotoxicity against human liver cancer cells - HepG2 (IC50 = 25 ± 0.5 µg mL-1), comparable to doxorubicin. Apoptotic features including membrane blebbing, chromatin condensation, and nuclear fragmentation were confirmed by Acridine Orange (AO/EB) dual staining and 4′,6-diamidino-2-phenylindole (DAPI) imaging. These findings establish green synthesized Cu/Cu2O-YSN as promising multifunctional nanomaterials for advanced biomedical applications.

Graphical Abstract