Scholarlink

<p>Neuropilin-1 (NRP-1) is overly expressed on the membrane of cervical cancer cells, which is indispensable for cell internalization. NRP-1 achieves the goal of targeting cervical cancer cells with tumor homing peptide (THPs) in C-terminal (CendR) pathway. By this feature, the ligand LyP-2 peptide of NRP-1 with high affinity (also containing CendR motif) was functionalized on the surface of liposome (Lipo@LyP-2) to mediate its tumor homing. Under TEM, Lipo@LyP-2 is a smooth global body, whose diameter was less than 200&#xa0;nm. Lipo@LyP-2 showed enhanced cell uptake in cervical cancer cells associated with human papillomavirus type 16 (HPV16) by the Confocal laser scanning microscope (CLSM). In the MTT assay, Lipo@LyP-2/SAHA/CDDP loaded with cisplatin (CDDP) and vorinostat (SAHA) also showed significant toxicity via increasing pro-apoptotic factors and decreasing anti-apoptotic factors. In addition, Western blot assay found that SAHA enhanced the intra-tumoral level of MHC class I chain-associated protein A (MICA) in cervical cancer, thus enhancing the sensitivity of cervical cancer cells to cytolysis mediated by natural killer (NK) cells. These results strongly indicate that LyP-2 functionalized Lipo/SAHA/CDDP can improve the targeting of CDDP and SAHA to SiHa cells and improve the therapeutic efficiency.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Treatment of Cervical Cancer with Tumor Homing Peptide LyP-2 Functionalized Liposome Loaded with Cisplatin and Vorinostat

  • 期刊论文

  • 发表日期：

    2025年5月21日

查看全文
  • Zuoping Li,
  • Jiaru Zhang,
  • Shiwan You,
  • Jing Zhang,
  • Zhenzhen Xie,
  • Na Zhao,
  • Shiguo Sun

摘要

Neuropilin-1 (NRP-1) is overly expressed on the membrane of cervical cancer cells, which is indispensable for cell internalization. NRP-1 achieves the goal of targeting cervical cancer cells with tumor homing peptide (THPs) in C-terminal (CendR) pathway. By this feature, the ligand LyP-2 peptide of NRP-1 with high affinity (also containing CendR motif) was functionalized on the surface of liposome (Lipo@LyP-2) to mediate its tumor homing. Under TEM, Lipo@LyP-2 is a smooth global body, whose diameter was less than 200 nm. Lipo@LyP-2 showed enhanced cell uptake in cervical cancer cells associated with human papillomavirus type 16 (HPV16) by the Confocal laser scanning microscope (CLSM). In the MTT assay, Lipo@LyP-2/SAHA/CDDP loaded with cisplatin (CDDP) and vorinostat (SAHA) also showed significant toxicity via increasing pro-apoptotic factors and decreasing anti-apoptotic factors. In addition, Western blot assay found that SAHA enhanced the intra-tumoral level of MHC class I chain-associated protein A (MICA) in cervical cancer, thus enhancing the sensitivity of cervical cancer cells to cytolysis mediated by natural killer (NK) cells. These results strongly indicate that LyP-2 functionalized Lipo/SAHA/CDDP can improve the targeting of CDDP and SAHA to SiHa cells and improve the therapeutic efficiency.