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<p>Oxidative stress is widely recognized as a vital factor in the etiopathogenesis of non-healing wounds. In our study, we propose an effective strategy that combines bone marrow mesenchymal stem cells (BMSCs) with an anti-oxidative scaffold fabricated by integrating salidroside and collagen, namely, the BMSCs-laden salidroside-collagen hybrid scaffold (BMSCs-SCOL). The fabricated SCOL scaffold exhibited suitable porosity, good water vapor transmission rate, successful drug release system, perfect cell adhesion and excellent cell viability for BMSCs. Moreover, the natural anti-oxidative compound salidroside showed in vitro rejuvenation effect on BMSCs by reducing the intracellular reactive oxygen species, increasing the mitochondrial membrane potential, reducing the activity of senescence-associated <i>β</i>-galactosidase (SA-<i>β</i>-gal) and downregulating the expressions of senescence-associated proteins, such as P21 and P16 in the D-gal induced model. In addition, the effect of BMSCs-SCOL on non-healing wounds was researched in vivo in Sprague–Dawley (SD) rats induced by full-thickness skin excision. Results showed that BMSCs-SCOL significantly promoted skin wound healing in SD rats, showing more collagen deposition and thinner epidermal thickness. BMSCs-SCOL also reduced the oxidative stress by decreasing malondialdehyde (MDA) content, increasing superoxide dismutase (SOD) activity and upregulating the expressions of collagen type 1 (COL-1), transforming growth factor beta1 (TGF-<i>β</i>1) and TGF-<i>β</i>3 in the wound tissues of SD rats. Therefore, BMSCs-SCOL represents a promising strategy to accelerate skin wound healing owing to its pronounced anti-oxidative properties and its ability to upregulate TGF-<i>β</i> expression.</p>

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Bone marrow mesenchymal stem cells-laden salidroside-collagen hybrid scaffold (BMSCs-SCOL) accelerates full-thickness skin wound healing by reducing oxidative stress and upregulating transforming growth factor beta

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Tong Zhu,
  • Hui-qin Li,
  • Ze-tai Zheng,
  • Tao Wang,
  • Yan-xin Zhou,
  • Ming-hua Li,
  • Wei Wu,
  • Xin-sheng Peng,
  • Yan-fang Zhou

摘要

Oxidative stress is widely recognized as a vital factor in the etiopathogenesis of non-healing wounds. In our study, we propose an effective strategy that combines bone marrow mesenchymal stem cells (BMSCs) with an anti-oxidative scaffold fabricated by integrating salidroside and collagen, namely, the BMSCs-laden salidroside-collagen hybrid scaffold (BMSCs-SCOL). The fabricated SCOL scaffold exhibited suitable porosity, good water vapor transmission rate, successful drug release system, perfect cell adhesion and excellent cell viability for BMSCs. Moreover, the natural anti-oxidative compound salidroside showed in vitro rejuvenation effect on BMSCs by reducing the intracellular reactive oxygen species, increasing the mitochondrial membrane potential, reducing the activity of senescence-associated β-galactosidase (SA-β-gal) and downregulating the expressions of senescence-associated proteins, such as P21 and P16 in the D-gal induced model. In addition, the effect of BMSCs-SCOL on non-healing wounds was researched in vivo in Sprague–Dawley (SD) rats induced by full-thickness skin excision. Results showed that BMSCs-SCOL significantly promoted skin wound healing in SD rats, showing more collagen deposition and thinner epidermal thickness. BMSCs-SCOL also reduced the oxidative stress by decreasing malondialdehyde (MDA) content, increasing superoxide dismutase (SOD) activity and upregulating the expressions of collagen type 1 (COL-1), transforming growth factor beta1 (TGF-β1) and TGF-β3 in the wound tissues of SD rats. Therefore, BMSCs-SCOL represents a promising strategy to accelerate skin wound healing owing to its pronounced anti-oxidative properties and its ability to upregulate TGF-β expression.