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<p>Uniformly dispersed CuO nanostructure thin films (CuO TF) were fabricated on FTO coated glass substrate using highly scalable, simple and cost-effective layer-by-layer dip-coating process. Surface uniformity, porosity and film thickness characterized using SEM micropscope, whereas XRD and Raman used to study the crystallographic phase, grain size and structural vibrational fingerprint of CuO TF. The electrochemical performance of the CuO TF electrode was systematically investigated for dual-mode anodic-glucose and cathodic-hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) non-enzymatic sensor. The optimized thickness CuO thin film exhibited an outstanding glucose sensing sensitivity of 4001 μAcm<sup>−2</sup>&#xa0;mM<sup>−1</sup> over a linear detection range of 5&#xa0;µM-325&#xa0;µM, with a low detection limit (LOD) of 7.5&#xa0;µM. The same CuO TF electrode demonstrated an outstanding activity towards H<sub>2</sub>O<sub>2</sub> with a sensitivity of 116 μAcm<sup>−2</sup>&#xa0;mM<sup>−1</sup>, a linear detection range of 0.25&#xa0;mM-10&#xa0;mM, and a low detection limit (LOD) of 25&#xa0;µM. The CuO TF also exhibited excellent electro-stability, selectivity, repeatability and reproducibility towards anodic-glucose and cathodic-H<sub>2</sub>O<sub>2</sub> sensing. The practical applicability of optimized CuO TF electrode was further validated by successfully detecting glucose in mountain dew, highlighting its potential as a scalable, cost-effective, enzyme-free and non-invasive platform for biomedical sensing.</p>

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Facile synthesis of uniformly dispersed CuO nanostructured for dual-mode non-enzymatic glucose and H2O2 sensing

  • 期刊论文

  • 发表日期：

    2026年6月23日

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  • Kaberi Saha,
  • Tanmoy Majumder,
  • Ankita Chakraborty,
  • Suvra Prakash Mondal,
  • Kamalesh Debnath

摘要

Uniformly dispersed CuO nanostructure thin films (CuO TF) were fabricated on FTO coated glass substrate using highly scalable, simple and cost-effective layer-by-layer dip-coating process. Surface uniformity, porosity and film thickness characterized using SEM micropscope, whereas XRD and Raman used to study the crystallographic phase, grain size and structural vibrational fingerprint of CuO TF. The electrochemical performance of the CuO TF electrode was systematically investigated for dual-mode anodic-glucose and cathodic-hydrogen peroxide (H2O2) non-enzymatic sensor. The optimized thickness CuO thin film exhibited an outstanding glucose sensing sensitivity of 4001 μAcm−2 mM−1 over a linear detection range of 5 µM-325 µM, with a low detection limit (LOD) of 7.5 µM. The same CuO TF electrode demonstrated an outstanding activity towards H2O2 with a sensitivity of 116 μAcm−2 mM−1, a linear detection range of 0.25 mM-10 mM, and a low detection limit (LOD) of 25 µM. The CuO TF also exhibited excellent electro-stability, selectivity, repeatability and reproducibility towards anodic-glucose and cathodic-H2O2 sensing. The practical applicability of optimized CuO TF electrode was further validated by successfully detecting glucose in mountain dew, highlighting its potential as a scalable, cost-effective, enzyme-free and non-invasive platform for biomedical sensing.