<p>This study investigates the mediating role of emotion regulation in the association between differentiation of self and marital conflict among married individuals. Employing a correlational design, the study recruited a sample of 250 married adults residing in Neyshabur (Iran) through convenience sampling. Data collection utilized the Kansas Marital Conflict Questionnaire, the Differentiation of Self Inventory, and the Emotion Regulation Questionnaire. Pearson correlation coefficients and path analysis assessed the hypothesized relationships. The findings revealed a significant association between differentiation of self and marital conflicts, mediated by emotion regulation. This indicates that emotion regulation plays a crucial role in the connection between these two variables. This research highlights the significance of emotion regulation in the relationship between the differentiation of self and marital conflict for married couples. The results provide valuable insights for family and couple therapists to consider these variables while developing intervention models and treatment approaches. The implications of this study suggest that enhancing emotion regulation skills in individuals may lead to reduced marital conflict, ultimately fostering healthier relationships.</p>