<p>In the United States, the Latinx college student population, such as those of Mexican descent, is growing and faces mental health disparities. One potential explanation for this may lie in how they navigate two cultures and, hence, endorse values to different extents than their caregivers, which may be associated with increased conflict. This study examined the associations between traditional gender roles (TGR), child-caregiver intergenerational acculturative conflict (IAC), and depressive symptoms among Mexican descent college students. It was hypothesized that IAC would mediate the association between TGRs and depressive symptoms. A total of 425 college students were recruited and completed a survey that assessed the variables of interest. For men, higher machismo levels were associated with less mother IAC and more father IAC, mother IAC mediated the relationship between caballerismo and depressive symptoms, and mother and father IAC mediated the association between machismo and depressive symptoms. For women, higher levels of self-silencing were associated with increased father IAC and decreased mother IAC, higher levels of family pillar were associated with higher mother IAC and less father IAC, higher levels of virtuous and chaste were associated with decreased father conflict, and both mother and father IAC mediated the relationship between self-silencing and depressive symptoms, and between family pillar and depressive symptoms. IAC was associated with depressive symptoms. These findings advance the understanding of these associations among this group. Research in this area is of importance as it furthers the understanding of factors associated with depressive symptoms and can inform prevention strategies for Mexican descent college students.</p>