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Purpose <p>To evaluate how the timing of oocyte denudation (DN) and intracytoplasmic sperm injection (ICSI) affects embryological and clinical outcomes in highly standardized oocyte donation cycles.</p> Methods <p>This retrospective cohort study included 1538 donor stimulation cycles from a single IVF center between January 2019 and July 2023. Timing intervals between human chorionic gonadotropin (hCG) administration, oocyte pick-up (OPU), DN, and ICSI were recorded. Outcomes included oocyte maturation (MII rate), fertilization, embryo quality, and clinical results. Statistical analyses included linear and logistic regression models, and chi-square tests, adjusting for donor and recipient age and BMI.</p> Results <p>Longer hCG-DN intervals (&gt; 39&#xa0;h) were significantly associated with increased MII rates and decreased proportions of MI and discarded oocytes (<i>P</i> &lt; 0.05). However, hCG-DN timing had no effect on fertilization rates, embryo quality, or pregnancy outcomes. Shorter DN-ICSI intervals (&lt; 90&#xa0;min) were associated with a higher proportion of top-quality blastocysts and fewer medium-quality embryos (<i>P</i> &lt; 0.05). DN-ICSI timing did not influence clinical outcomes. Recipient BMI was the strongest predictor of pregnancy, clinical pregnancy, and live birth (<i>P</i> &lt; 0.001).</p> Conclusions <p>Extended incubation prior to denudation improves oocyte maturation but does not affect fertilization or pregnancy. Short DN-ICSI intervals favor top-quality blastocyst formation without altering clinical results. Recipient BMI significantly impacts reproductive success. These findings support timing optimization in ART and highlight the influence of patient-specific factors.</p>

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Examining the impact of denudation and ICSI timing on embryological and clinical outcomes in oocyte donation cycles

  • 期刊论文

  • 发表日期：

    2025年12月8日

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  • Sergi Novo,
  • Marc Yeste,
  • Lurdes Martiñá,
  • Fidalma Boninu,
  • Sergi Rovira,
  • Federica Moffa,
  • Marta Antich

摘要

Purpose

To evaluate how the timing of oocyte denudation (DN) and intracytoplasmic sperm injection (ICSI) affects embryological and clinical outcomes in highly standardized oocyte donation cycles.

Methods

This retrospective cohort study included 1538 donor stimulation cycles from a single IVF center between January 2019 and July 2023. Timing intervals between human chorionic gonadotropin (hCG) administration, oocyte pick-up (OPU), DN, and ICSI were recorded. Outcomes included oocyte maturation (MII rate), fertilization, embryo quality, and clinical results. Statistical analyses included linear and logistic regression models, and chi-square tests, adjusting for donor and recipient age and BMI.

Results

Longer hCG-DN intervals (> 39 h) were significantly associated with increased MII rates and decreased proportions of MI and discarded oocytes (P < 0.05). However, hCG-DN timing had no effect on fertilization rates, embryo quality, or pregnancy outcomes. Shorter DN-ICSI intervals (< 90 min) were associated with a higher proportion of top-quality blastocysts and fewer medium-quality embryos (P < 0.05). DN-ICSI timing did not influence clinical outcomes. Recipient BMI was the strongest predictor of pregnancy, clinical pregnancy, and live birth (P < 0.001).

Conclusions

Extended incubation prior to denudation improves oocyte maturation but does not affect fertilization or pregnancy. Short DN-ICSI intervals favor top-quality blastocyst formation without altering clinical results. Recipient BMI significantly impacts reproductive success. These findings support timing optimization in ART and highlight the influence of patient-specific factors.