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Purpose <p>The efficacy of oocyte cryopreservation still requires enhancement. Current understanding of cryodamage is largely limited to the organelle level, and the impact of freezing on oocyte gene expression remains unclear.</p> Methods <p>In this study, we employed an innovative dual-omics approach to assess the transcriptional and translational profiles of mouse metaphase II (MII) oocytes during the initial 4 h post-thaw.</p> Results <p>Our mapping of the translational recovery in vitrified mouse oocytes post-thaw revealed a critical 2-h window that is optimal for recovery. We confirmed the mitochondrial damage associated with vitrification and identified the activation of autophagy and proteasomal degradation during this period. Additionally, our analysis indicates that vitrified oocytes have another repair response to counteract cryoinjury, involving spindle remodeling and membrane recycling.</p> Conclusions <p>These findings can guide future efforts to improve oocyte vitrification outcomes by improving repair processes and not focusing solely on the damage processes.</p>

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Dual-omics reveals temporal translational recovery landscapes and cryodamage repair mechanisms in vitrified mouse oocytes

  • 期刊论文

  • 发表日期：

    2025年6月10日

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  • Shaohong Zhuang,
  • Xiaoting Diao,
  • Rui Xiang,
  • Jiana Huang,
  • Jiahui Pang,
  • Na Lu,
  • Qiqi Liang,
  • Xinyi Pan,
  • Rang Liu,
  • Cong Fang,
  • Xiaoyan Liang,
  • Wenlin Peng,
  • Haitao Zeng

摘要

Purpose

The efficacy of oocyte cryopreservation still requires enhancement. Current understanding of cryodamage is largely limited to the organelle level, and the impact of freezing on oocyte gene expression remains unclear.

Methods

In this study, we employed an innovative dual-omics approach to assess the transcriptional and translational profiles of mouse metaphase II (MII) oocytes during the initial 4 h post-thaw.

Results

Our mapping of the translational recovery in vitrified mouse oocytes post-thaw revealed a critical 2-h window that is optimal for recovery. We confirmed the mitochondrial damage associated with vitrification and identified the activation of autophagy and proteasomal degradation during this period. Additionally, our analysis indicates that vitrified oocytes have another repair response to counteract cryoinjury, involving spindle remodeling and membrane recycling.

Conclusions

These findings can guide future efforts to improve oocyte vitrification outcomes by improving repair processes and not focusing solely on the damage processes.