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Purpose <p>Semen manipulation for assisted reproductive technology (ART) causes spermatozoa damage; thus, we investigated the potential of the novel therapeutic BGP-15 to preserve sperm quality during semen washing prior to insemination.</p> Methods <p>Donated human ejaculates (<i>N</i> = 40), with or without 10&#xa0;µM BGP-15, were analyzed for sperm motility, DNA fragmentation, and oxidation. Seminal plasma was removed using different clinical sperm selection methods: simple wash, swim-up, or density gradient centrifugation (DGC), followed by assessment for sperm motility, mitochondrial ROS (mtROS), mitochondrial membrane potential (MMP), and DNA fragmentation and oxidation.</p> Results <p>Donated semen samples incubated with BGP-15 had increased sperm motility (+ 15%, <i>p</i> = 0.002) and reduced oxidative DNA damage levels (− 57%, <i>p</i> = 0.03).</p> <p>Samples processed by simple wash had the highest sperm count compared with DGC (+ 55%, <i>p</i> &lt; 0.005) and swim-up (+ 21%, <i>p</i> &lt; 0.0005). Swim-up showed increased vitality compared with DGC (+ 18%, <i>p</i> &lt; 0.001) and simple wash (+ 27%, <i>p</i> &lt; 0.0001), as well as the lowest DNA oxidation levels compared with simple wash − 40%, (<i>p</i> = 0.01) and DGC (− 76%, <i>p</i> &lt; 0.0001). Swim-up also had the lowest mitochondrial membrane potential compared with simple wash and DGC (− 28%, <i>p</i> &lt; 0.03).</p> <p>Comparison between untreated and BGP-15-treated groups for each sperm washing method showed that BGP-15 increased MMP in DGC sperm (+ 11%, <i>p</i> = 0.0006), and reduced DNA fragmentation in washed samples (− 22%, <i>p</i> = 0.03). Moreover, BGP-15 lowered DNA oxidation in all preparation methods: washed (− 48%, <i>p</i> = 0.002), swim-up (− 42%, <i>p</i> = 0.04), and DGC (− 29%, <i>p</i> &lt; 0.0001).</p> Conclusions <p>The inclusion of BGP-15 during semen preparation can protect sperm quality and, in the future, may be used clinically to improve sperm selection methods.</p>

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Sperm oxidative damage acquired during seminal plasma removal for assisted reproductive technology is reduced by BGP-15

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年2月11日

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  • Macarena B. Gonzalez,
  • Ryan D. Rose,
  • Haley S. Connaughton,
  • Gracie Mackintosh,
  • Caitlyn Bugeja,
  • Michael Barry,
  • Nicole O. McPherson,
  • Rebecca L. Robker

摘要

Purpose

Semen manipulation for assisted reproductive technology (ART) causes spermatozoa damage; thus, we investigated the potential of the novel therapeutic BGP-15 to preserve sperm quality during semen washing prior to insemination.

Methods

Donated human ejaculates (N = 40), with or without 10 µM BGP-15, were analyzed for sperm motility, DNA fragmentation, and oxidation. Seminal plasma was removed using different clinical sperm selection methods: simple wash, swim-up, or density gradient centrifugation (DGC), followed by assessment for sperm motility, mitochondrial ROS (mtROS), mitochondrial membrane potential (MMP), and DNA fragmentation and oxidation.

Results

Donated semen samples incubated with BGP-15 had increased sperm motility (+ 15%, p = 0.002) and reduced oxidative DNA damage levels (− 57%, p = 0.03).

Samples processed by simple wash had the highest sperm count compared with DGC (+ 55%, p < 0.005) and swim-up (+ 21%, p < 0.0005). Swim-up showed increased vitality compared with DGC (+ 18%, p < 0.001) and simple wash (+ 27%, p < 0.0001), as well as the lowest DNA oxidation levels compared with simple wash − 40%, (p = 0.01) and DGC (− 76%, p < 0.0001). Swim-up also had the lowest mitochondrial membrane potential compared with simple wash and DGC (− 28%, p < 0.03).

Comparison between untreated and BGP-15-treated groups for each sperm washing method showed that BGP-15 increased MMP in DGC sperm (+ 11%, p = 0.0006), and reduced DNA fragmentation in washed samples (− 22%, p = 0.03). Moreover, BGP-15 lowered DNA oxidation in all preparation methods: washed (− 48%, p = 0.002), swim-up (− 42%, p = 0.04), and DGC (− 29%, p < 0.0001).

Conclusions

The inclusion of BGP-15 during semen preparation can protect sperm quality and, in the future, may be used clinically to improve sperm selection methods.