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<p>A perylene-based turn-off fluorescent strategy for tetracycline antibiotics (TCs) detection has been established with gold nanoparticles. TCs directly participate in the reduction of chloroauric acid to generate gold atoms, which subsequently nucleate and assemble into gold nanoparticles (TCs–AuNPs). Because of the overlap between the absorption of TCs–AuNPs and the fluorescence emission of the perylene probe (PDI), the inner filter effect (IFE)-based fluorescence turn-off assay was successfully established. Consequently, the fluorescence peaks of the PDI at 550 and 591 nm were quenched by TC–AuNPs via the IFE process. The assay method shows high sensitivity with a limit of detection of 2.4 mM, and remarkable selectivity against common interfering substances. The turn-off fluorescence sensing method is fairly simple, fast, and inexpensive, and it has also been successfully used for real water sample analysis. Consequently, a highly sensitive and selective method for the detection of TC via a fluorescent turn-off mechanism based on the IFE between the PDI probe and TC–AuNPs in aqueous media was successfully established.</p>

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Perylene-Based Turn-Off Fluorescent Probe for Tetracycline Antibiotics Detection with Gold Nanoparticles

  • 期刊论文

  • 发表日期：

    2026年7月21日

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  • Y. X. Wu,
  • Q. Wang,
  • T. D. Rao,
  • M. Y. Huang,
  • L. J. Liang,
  • H. C. Li

摘要

A perylene-based turn-off fluorescent strategy for tetracycline antibiotics (TCs) detection has been established with gold nanoparticles. TCs directly participate in the reduction of chloroauric acid to generate gold atoms, which subsequently nucleate and assemble into gold nanoparticles (TCs–AuNPs). Because of the overlap between the absorption of TCs–AuNPs and the fluorescence emission of the perylene probe (PDI), the inner filter effect (IFE)-based fluorescence turn-off assay was successfully established. Consequently, the fluorescence peaks of the PDI at 550 and 591 nm were quenched by TC–AuNPs via the IFE process. The assay method shows high sensitivity with a limit of detection of 2.4 mM, and remarkable selectivity against common interfering substances. The turn-off fluorescence sensing method is fairly simple, fast, and inexpensive, and it has also been successfully used for real water sample analysis. Consequently, a highly sensitive and selective method for the detection of TC via a fluorescent turn-off mechanism based on the IFE between the PDI probe and TC–AuNPs in aqueous media was successfully established.