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<p>A fluorescence spectroscopy study has been carried out on the interaction of Hoechst 33258 (H33258) with single-stranded (ss-)polyribonucleotides poly(rA) and poly(rU) and double-stranded (ds)-poly(rA)–poly(rU). Groove-binding compound H33258, which is a specific ligand for ds-nucleic acids (ds-NA), can also bind to ss-RNA. H33258 shows higher preference for poly(rA)–poly(rU) formed due to hybridization than for ss-poly(rA) and ss-poly(rU). Fluorescence spectroscopy was used to construct the binding curves of H33258 to ss- and dspolyribonucleotides. The binding constants K and number of bases per binding site n were determined. The data obtained indicated that the binding constant of H33258 to ss-poly(rA) is 4–5 times higher than that for sspoly(rU). The affinity of H33258 for poly(rA)–poly(rU) and ss-poly(rA) was found to depend on the ionic strength of the solution, while such a dependence is not found for ss-poly(rU).</p>

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Fluorescence Characteristics of Hoechst 33258 Complexes with Single-Stranded and Double-Stranded Polyribonucleotides in Solution at Various Solution Ionic Strengths

  • 期刊论文

  • 发表日期：

    2025年1月16日

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  • A. P. Antonyan,
  • P. O. Vardevanyan,
  • M. S. Mikaelyan,
  • G. H. Poghosyan,
  • G. H. Kocharyan,
  • M. A. Parsadanyan

摘要

A fluorescence spectroscopy study has been carried out on the interaction of Hoechst 33258 (H33258) with single-stranded (ss-)polyribonucleotides poly(rA) and poly(rU) and double-stranded (ds)-poly(rA)–poly(rU). Groove-binding compound H33258, which is a specific ligand for ds-nucleic acids (ds-NA), can also bind to ss-RNA. H33258 shows higher preference for poly(rA)–poly(rU) formed due to hybridization than for ss-poly(rA) and ss-poly(rU). Fluorescence spectroscopy was used to construct the binding curves of H33258 to ss- and dspolyribonucleotides. The binding constants K and number of bases per binding site n were determined. The data obtained indicated that the binding constant of H33258 to ss-poly(rA) is 4–5 times higher than that for sspoly(rU). The affinity of H33258 for poly(rA)–poly(rU) and ss-poly(rA) was found to depend on the ionic strength of the solution, while such a dependence is not found for ss-poly(rU).