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<p>A novel approach to the isolation of commercially valuable carotenoids from cyanobacteria is suggested. The protocol avoids the application of costly column chromatography. As an example, the isolation of myxoxanthophyll and oscillaxanthin from <i>Limnospira (Spirulina) platensis</i> (Gomont) K.R.S.Santos &amp; Hentschke is described. The method is based on the surface-active properties of a unique carotenoid-lipoprotein complex, which accumulates at the chloroform-water interface and can be easily extracted. The complex is composed of myxol 2'-α-L-quinovoside, oscillol 2,2'-di-α-L-quinovoside, and lipoproteins. The mass fraction of carotenoids in the complex was 23 ± 7%. The ratio of the carotenoids in the complex was 63 ± 9% myxoxanthophyll to 27 ± 7% oscillaxanthin. The carotenoids were identified using ultraviolet and visible (UV–Vis) absorption spectroscopy, HPLC analysis, fast atom bombardment mass spectrometry (FAB MS), and proton magnetic resonance spectrometry (<sup>1</sup>H NMR). The complex was characterized using vibrational infrared spectroscopy (FTIR). Myxoxanthophyll and oscillaxanthin yield from a dry sample of <i>L. platensis</i> was 0.11 ± 0.03% and 0.05 ± 0.02%, respectively, with 40&#xa0;mg of myxoxanthophyll and 17&#xa0;mg of oscillaxanthin being isolated from 36.84&#xa0;g of <i>L. platensis</i> (dry weight). After the carotenoid isolation, further extraction of water-soluble phycobilins from the residual biomass is possible.</p> Graphical abstract <p></p>

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Facile isolation of lipoprotein-bound myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis

  • 期刊论文

  • 发表日期：

    2026年8月3日

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  • R. G. Gevorgiz,
  • A. A. Gontcharov,
  • S. R. Abdullin,
  • A. Y. Nikulin,
  • L. L. Kapranova,
  • S. N. Zheleznova,
  • A. A. Blaginina,
  • T. A. Telegina,
  • Y. L. Vechtomova,
  • T. Maoka,
  • M. V. Nekhoroshev

摘要

A novel approach to the isolation of commercially valuable carotenoids from cyanobacteria is suggested. The protocol avoids the application of costly column chromatography. As an example, the isolation of myxoxanthophyll and oscillaxanthin from Limnospira (Spirulina) platensis (Gomont) K.R.S.Santos & Hentschke is described. The method is based on the surface-active properties of a unique carotenoid-lipoprotein complex, which accumulates at the chloroform-water interface and can be easily extracted. The complex is composed of myxol 2'-α-L-quinovoside, oscillol 2,2'-di-α-L-quinovoside, and lipoproteins. The mass fraction of carotenoids in the complex was 23 ± 7%. The ratio of the carotenoids in the complex was 63 ± 9% myxoxanthophyll to 27 ± 7% oscillaxanthin. The carotenoids were identified using ultraviolet and visible (UV–Vis) absorption spectroscopy, HPLC analysis, fast atom bombardment mass spectrometry (FAB MS), and proton magnetic resonance spectrometry (1H NMR). The complex was characterized using vibrational infrared spectroscopy (FTIR). Myxoxanthophyll and oscillaxanthin yield from a dry sample of L. platensis was 0.11 ± 0.03% and 0.05 ± 0.02%, respectively, with 40 mg of myxoxanthophyll and 17 mg of oscillaxanthin being isolated from 36.84 g of L. platensis (dry weight). After the carotenoid isolation, further extraction of water-soluble phycobilins from the residual biomass is possible.

Graphical abstract